Pathological analysis of PAD and citrullinated proteins that triggers the onset of Alzheimer's disease

触发阿尔茨海默病发作的 PAD 和瓜氨酸蛋白的病理学分析

• 批准号: 21590392
• 负责人: HANDA Setsuko
• 金额: $ 3万
• 依托单位: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590392/
• 关键词: 脳・神経; アルツハイマー病; シトルリン化; PAD; プロテオーム; 診断薬

The abnormal accumulation of citrullinated proteins were detected in the brain from Alzheimer's disease(AD) patients. By using two-dimensional gel electrophoresis and MALDI-TOF mass spectrometry, two of the citrullinated proteins were identified as a vimentin and a glial fibrillary acidic protein(GFAP). In addition, we constructed a ELISA system which detect the citrullinated GFAP to develop diagnostic laboratory tests for early diagnosis of AD.

阿尔茨海默病（AD）患者脑内存在瓜氨酸化蛋白的异常积累。通过使用二维凝胶电泳和MALDI-TOF质谱，两个瓜氨酸化蛋白被鉴定为波形蛋白和胶质细胞酸性蛋白（GFAP）。此外，我们还建立了瓜氨酸化胶质纤维酸性蛋白的ELISA检测体系，以建立AD早期诊断的实验室诊断方法。

项目成果

Elevated levels of 4-hydroxynonenal-histidine Michael adduct in the hippocampi of patients with Alzheimer's disease

• DOI: 10.2220/biomedres.30.227
• 发表时间: 2009-08-01
• 期刊: BIOMEDICAL RESEARCH-TOKYO
• 影响因子: 1.2
• 作者: Fukuda, Mitsugu;Kanou, Fumihisa;Ishigami, Akihito
• 通讯作者: Ishigami, Akihito

Subcellular Localization of Peptidylarginine Deiminase 2 and Citrullinated Proteins in Brains of Scrapie-Infected Mice: Nuclear Localization of PAD2 and Membrane Fraction-Enriched Citrullinated Proteins

• DOI: 10.1097/nen.0b013e318207559e
• 发表时间: 2011-02-01
• 期刊: JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
• 影响因子: 3.2
• 作者: Jang, Byungki;Shin, Hae-Young;Choi, Eun-Kyoung
• 通讯作者: Choi, Eun-Kyoung

Deimination and expression of peptidylarginine deiminases during cutaneous wound healing in mice

• DOI: 10.1684/ejd.2011.1394
• 发表时间: 2011-05-01
• 期刊: EUROPEAN JOURNAL OF DERMATOLOGY
• 影响因子: 2.5
• 作者: Coudane, Fanny;Mechin, Marie-Claire;Simon, Michel
• 通讯作者: Simon, Michel

関節リウマチにおける血漿ペプチジルアルギニンデイミナーゼ4(PAD4)及び抗PAD4抗体

类风湿关节炎中的血浆肽精氨酸脱亚胺酶 4 (PAD4) 和抗 PAD4 抗体

• 发表时间: 2009
• 作者: 木村直樹;杉原毅彦;石神昭人
• 通讯作者: 石神昭人

Peptidylarginine deiminase modulates the physiological roles of enolase via citrullination: links between altered multifunction of enolase and neurodegenerative diseases

• DOI: 10.1042/bj20120025
• 发表时间: 2012-07-15
• 期刊: BIOCHEMICAL JOURNAL
• 影响因子: 4.1
• 作者: Jang, Byungki;Jeon, Yong-Chul;Choi, Eun-Kyoung
• 通讯作者: Choi, Eun-Kyoung