The use of novel humanized mouse model for the study of immunotherapy against HTLV-1 infection

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In this study, HTLV-1-negative normal human peripheral blood mononuclear cells(PBMCs) were transplanted directly into the spleens of NOD/SCID/γCnull(NOG) mice together with mitomycin treated HTLV-1-infected cell line. We demonstrated that the both human CD4 and CD8 positive T cells were infected with HTLV-1, and that Tax expression was induced by short time(6hrs) in vitro cultivation, as observed in naturally infected T-cells. Moreover, viral gene expression was also very similar to those observed in naturally infected T-cells. Our method provides a simple, rapid and effective approach for assessment of HTLV-1 infection in vivo ; it is therefore useful in the study of infection, pathogenesis and immunotherapies.