Identification of liver disease-specific peptide by sugar chain profiling

点击翻译按钮获取中文摘要

We identified inter-α-trypsin inhibitor heavy chain 4(ITIH4) peptide as a novel serum biomarker for liver disease using 2D-μHPLC-MALDI-TOF-MS. Also we developed immuno-MS system to deal with many samples. Using this system, we determined the utility of this biomarker peptide for detecting liver cirrhosis and hepatocellular carcinoma(HCC). Moreover, this peptide was useful for detecting non-alcoholic fatty liver diseases. And 35kD protein fragment including this peptide was divided into ten isoforms by the sugar chain modification, and serum isofrom 9 and 10 levels were elevated in early HCC patients. These isoforms are useful biomarker for HCC and may be related to hepatic carcinogenesis.