In Vitro Behavior of Hepatitis C Virus JFH-1 Strains with Mutations Emerged after Passage in Chimpanzees

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Although JFH-1 replicates efficiently and produces infectious virus in cell culture, the relationship between in vitro phenotype and in vivo pathogenesis is still unclear. We synthesized the full-genome JFH-1 constructs with mutations detected in chimpanzees and assessed effects of these mutations on viral production in vitro. Higher virus assembly and lower replication capacity of JFH-1 with these mutations were observed. The control of viral production such as replicati on, assembly and secretion by specific mutations may be a key of viral strategy to evade host defense mechanisms.