In Vitro Behavior of Hepatitis C Virus JFH-1 Strains with Mutations Emerged after Passage in Chimpanzees

丙型肝炎病毒 JFH-1 株在黑猩猩传代后出现突变的体外行为

• 批准号: 21590868
• 负责人: KATO Takanobu
• 金额: $ 2.91万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590868/
• 关键词: HCV; 慢性化; ウイルス培養; 感染実験; チンパンジー; 慢性感染; 動物モデル; ウイルス増殖モデル; ウイルス培養実験

Although JFH-1 replicates efficiently and produces infectious virus in cell culture, the relationship between in vitro phenotype and in vivo pathogenesis is still unclear. We synthesized the full-genome JFH-1 constructs with mutations detected in chimpanzees and assessed effects of these mutations on viral production in vitro. Higher virus assembly and lower replication capacity of JFH-1 with these mutations were observed. The control of viral production such as replicati on, assembly and secretion by specific mutations may be a key of viral strategy to evade host defense mechanisms.

尽管JFH-1在细胞培养中高效复制并产生感染性病毒，但体外表型与体内发病机制之间的关系仍不清楚。我们合成了在黑猩猩中检测到突变的全基因组JFH-1构建体，并评估了这些突变对体外病毒产生的影响。观察到具有这些突变的JFH-1的更高的病毒组装和更低的复制能力。通过特定突变控制病毒的复制、组装和分泌等生产可能是病毒逃避宿主防御机制的关键策略。

项目成果

C型肝炎ウイルスJFH-1株患者血清のチンパンジーへの感染実験

丙型肝炎病毒JFH-1患者血清对黑猩猩的感染实验

• 发表时间: 2010
• 作者: 加藤孝宣;Saeed Mohsan,脇田隆字
• 通讯作者: Saeed Mohsan,脇田隆字

Development of recombinant hepatitis C virus with NS5A from strains of genotypes 1 and 2

• DOI: 10.1016/j.bbrc.2011.05.144
• 发表时间: 2011-07-08
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Okamoto, Yuka;Masaki, Takahiro;Kato, Takanobu
• 通讯作者: Kato, Takanobu

Studies on virus kinetics using infectious fluorescence-tagged hepatitis C virus cell culture

• DOI: 10.1111/j.1872-034x.2010.00771.x
• 发表时间: 2011-03-01
• 期刊: HEPATOLOGY RESEARCH
• 影响因子: 4.2
• 作者: Yamamoto, Machi;Sakamoto, Naoya;Watanabe, Mamoru
• 通讯作者: Watanabe, Mamoru

Hepatitis C Virus JFH-1 Strain That Adapted In Vivo Acquired Abilities for Efficient Virus Production and Anti-apoptosis

体内适应的丙型肝炎病毒JFH-1株获得了高效病毒生产和抗凋亡的能力

• 发表时间: 2010
• 作者: M Saeed;他
• 通讯作者: 他

In vitro behavior of hepatitis C virus JFH-1 strains with mutations emerged after passage in chimpanzees

带有突变的丙型肝炎病毒JFH-1株在黑猩猩传代后出现的体外行为

• 发表时间: 2009
• 作者: Mohsan Saeed;Takanobu Kato;Youkyung Choi;Krzyaztof Krawczynski;Jake Liang;Takaji Wakita
• 通讯作者: Takaji Wakita