A new treatment for arrhythmia with ghrelin via the modulation of autonomic nerve activity

生长素释放肽通过调节自主神经活动治疗心律失常的新方法

• 批准号: 21590899
• 负责人: SOEKI Takeshi
• 金额: $ 2.91万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590899/
• 关键词: 循環器・高血圧; 生理活性; 薬理学; 心臓病; 生理活性ペプチド

Male Sprague-Dawley rats were exposed to a 30 minutes of ischemia following ligation of the left coronary artery. Animals were then randomized to receive either ghrelin or saline during the period of coronary ligation. Power spectral analysis of heart rate variability revealed that the administration of ghrelin increased the high-frequency(HF) power and decreased the low-frequency(LF)/ HF ratio. Ventricular tachyarrhythmias were less frequent in rats after MI who received ghrelin in comparison to rats who received saline. Immunoblotting and immunohistovhemistry revealed that rats given saline alone during MI exhibited a marked reduction in phosphorylated connexin 43 within the left ventricle, whereas those that received ghrelin displayed only minor reductions in comparison to sham-operated rats. These effects of ghrelin were diminished by the co-administration of atropine or the blockade of vagal afferents.

在结扎左冠状动脉后，将雄性Sprague-Dawley大鼠暴露于30分钟的局部缺血。然后在冠状动脉结扎期间将动物随机接受生长激素释放肽或盐水。心率变异性的功率谱分析显示，ghrelin的管理增加了高频（HF）的功率，降低了低频（LF）/ HF的比例。与接受生理盐水的大鼠相比，接受生长激素释放肽的MI后大鼠的室性快速性心律失常发生率较低。免疫印迹和荧光分析显示，在MI期间仅给予生理盐水的大鼠在左心室内磷酸化连接蛋白43显着减少，而接受ghrelin的大鼠与假手术大鼠相比仅显示轻微减少。这些影响ghrelin减少了共同管理的阿托品或迷走神经传入阻滞。

项目成果

グレリンによる自律神経およびConnexin43発現調節を介した抗不整脈作用の検討

通过调节自主神经和 Connexin43 表达检查 ghrelin 的抗心律失常作用

• 发表时间: 2011
• 作者: Washio;I.;坂東左知子
• 通讯作者: 坂東左知子

Ghrelin Protects Heart Against Ischemia-Induced Arrhythmias by Preserving Connexin43 Protein

Ghrelin 通过保留 Connexin43 蛋白保护心脏免受缺血引起的心律失常

• 发表时间: 2010
• 作者: 添木武;他
• 通讯作者: 他