Transplantation of allogenic fetal membrane-derived mesenchymal stem cells protect kidney

同种异体胎膜间充质干细胞移植保护肾脏

• 批准号: 21591028
• 负责人: ISAKA Yoshitaka
• 金额: $ 3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591028/
• 关键词: 幹細胞; 移植; 腎炎; 虚血再灌流傷害; 再生; 間葉系幹細胞移植; 再生医療; 卵膜由来幹細胞; 尿細管細胞; 間葉系線細胞移植; サイトカイン; 虚血再還流傷害

Mesenchymal stem cell(MSC) is an attractive therapeutic cell source for treating renal diseases. MSC administration has been shown to improve renal function, although underlying mechanisms are incompletely understood. We recently showed that allogenic fetal membrane-derived MSC(FM-MSC), which is available non-invasively in large amounts, had renoprotective effect in an experimental glomerulonephritis model. Here, we investigated whether allogenic FM-MSC administration could protect kidneys from anti-Thy-1 glomerulonephritis or ischemia/ reperfusion(I/ R) injury. Histological examination revealed that FM-MSC administration significantly suppressed glomerular injury or I/ R injury. Administered FM-MSC were rarely observed in kidneys, but administration of FM-MSC increased serum IL-10 levels. In addition, renoprotective effects of FM-MSC were abolished by using anti-IL-10 neutralization antibody, suggesting that IL-10 would be one of the candidate factors to protect rat kidney from kidney injury. We concluded that allogenic FM-MSC transplantation is a potent therapeutic strategy for the treatment of kidney diseases.

间充质干细胞(MSC)是一种很有吸引力的治疗肾脏疾病的细胞来源。MSC的应用已被证明可以改善肾功能，尽管其潜在的机制尚不完全清楚。我们最近发现，大量非侵入性的同种异体胎膜来源的间充质干细胞(FM-MSC)在实验性肾小球肾炎模型中具有肾脏保护作用。在这里，我们研究了同种异体FM-MSC给药是否能保护肾脏免受抗Thy-1肾炎或缺血/再灌注(I/R)损伤。组织学检查显示，FM-MSC可明显抑制肾小球损伤或I/R损伤。应用FM-MSC在肾脏中很少观察到，但应用FM-MSC可增加血清IL-10水平。此外，FM-MSC的肾脏保护作用可被抗IL-10中和抗体阻断，提示IL-10可能是保护大鼠肾脏损伤的候选因子之一。我们认为同种异体骨髓间充质干细胞移植是治疗肾脏疾病的有效治疗策略。

项目成果

Allogenic fetal membrane-derived mesenchymal stem cells contribute to renal repair in experimental glomerulonephritis

• DOI: 10.1152/ajprenal.00587.2009
• 发表时间: 2010-11-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
• 影响因子: 4.2
• 作者: Tsuda, Hidetoshi;Yamahara, Kenichi;Ikeda, Tomoaki
• 通讯作者: Ikeda, Tomoaki