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精神機能に及ぼす甲状腺ホルモン作用におけるRCAN/カルシニューリン系の役割

RCAN/钙调神经磷酸酶系统在甲状腺激素对精神功能的作用中的作用

基本信息

批准号：
21591173
负责人：
KANOU Yasuhiko
金额：
$ 2.83万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2012
项目状态：
已结题

项目摘要

RCAN (regulators of calcineurin) 2 is induced with a thyroxin (T3) and it controls the activity of a calcineurin (CaN). CaN is a protein phosphatase which exists in a CNS at a rich, and it is reported that unusuallies arise in a learning and a memory, or a mentation by the knock out and overexpression. Since the expression of cerebral RCAN2 reduced due to the hypothyroidism, RCAN2 participated in the cerebral function deeply and we assumed that the effect to the cerebral function of a thyroxin was demonstrated through the regulation of gene expression of RCAN2. This study examined the role in the cerebral function of RCAN2 protein through the morphological and behavioral analyses of the previously established RCAN2 genetically engineered mouse (knock out mouse). A RCAN2 KO mice is born normally and the abnormality compared with a wild type was not observed. We established as a congenic strain to C57BL/6J line, which carried out the back, cross, and made the backcross generation's 10 to … More 11 (N10or11) F2 mice, and provided the behavioral analyze. Moreover, we deposited the mice to the Riken bioresource center. In a nascent, a growth period, and an adult, a morphological variation does not have a RCAN2KO mice in a brain compared with a wild type mice, and a future still more detailed investigation is called for. As a result of measuring the amount of behaviourals of a RCAN2 KO mice, the difference [superiority / the amount of spontaneous behaviourals of KO mice, a wild type mice, and a hetero type mice] was not accepted. However, the amount of behavioral of KO mice was decreasing immediately after moving to a novel situation (novel cage for a measurement). Furthermore, I considered the difference to be an out-of thing to the emotional behavior and learning ability that have been measured by other behavioral trials (an elevated type crux maze trial, Y character maze trial, a forced-swimming trial, phobia conditional learning tests). The analyze by the "Japan mouse clinic" which a Riken bioresource center carry out is under perform. Less

RCAN（钙调磷酸酶调节因子）2是由甲状腺素（T3）诱导的，它控制钙调磷酸酶（CaN）的活性。CaN是一种丰富存在于中枢神经系统的蛋白磷酸酶，据报道，在学习、记忆或精神状态中出现异常的敲除和过度表达。由于甲状腺功能减退导致脑RCAN2表达减少，RCAN2深度参与脑功能，我们认为a甲状腺素对脑功能的影响是通过调控RCAN2基因表达来证明的。本研究通过对先前建立的RCAN2基因工程小鼠（敲除小鼠）的形态学和行为学分析，研究了RCAN2蛋白在脑功能中的作用。RCAN2 KO小鼠出生正常，与野生型相比未见异常。以C57BL/6J系为遗传品系，进行回交、杂交，制得回交代10 ~ 11只（N10or11） F2小鼠，并进行行为学分析。此外，我们将小鼠存放在理研生物资源中心。在新生期、生长期和成年期，与野生型小鼠相比，形态变异的RCAN2KO小鼠的大脑中没有RCAN2KO，未来需要更详细的研究。通过测量RCAN2 KO小鼠的行为数量，发现KO小鼠、野生型小鼠和异种型小鼠自发行为的优势/数量的差异是不可接受的。然而，在移动到一个新的环境（新的笼子进行测量）后，KO小鼠的行为量立即下降。此外，我认为这种差异与其他行为试验（高难度迷宫试验、Y字迷宫试验、强迫游泳试验、恐惧症条件学习测试）所测量的情绪行为和学习能力是不相符的。理研生物资源中心开展的“日本老鼠诊所”的分析工作不尽如人意。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Inactivation of the Rcan2 gene in mice ameliorates the age- and diet-induced obesity by causing a reduction in food intake.

DOI：
10.1371/journal.pone.0014605
发表时间：
2011-01-27
期刊：
PloS one
影响因子：
3.7
作者：
Sun XY;Hayashi Y;Xu S;Kanou Y;Takagishi Y;Tang YP;Murata Y
通讯作者：
Murata Y

Chronic restraint stress impairs neurogenesis and hippocampus-dependent fear memory in mice: possible involvement of a brain-specific transcription factor Npas4

DOI：
10.1111/j.1471-4159.2010.06893.x
发表时间：
2010-09-01
期刊：
JOURNAL OF NEUROCHEMISTRY
影响因子：
4.7
作者：
Yun, Jaesuk;Koike, Hiroyuki;Yamada, Kiyofumi
通讯作者：
Yamada, Kiyofumi

Mice Deficient for Glucagon Gene-Derived Peptides Display Normoglycemia and Hyperplasia of Islet {alpha}-Cells But Not of Intestinal L-Cells.

缺乏胰高血糖素基因衍生肽的小鼠表现出血糖正常和胰岛 α 细胞增生，但肠 L 细胞则不然。

DOI：
发表时间：
2009
期刊：
Molecular Endocrinology
影响因子：
0
作者：
Hayashi Y;Yamamoto M;Mizoguchi H;Watanabe C;Ito R;Yamamoto S;Sun XY;and Murata Y.
通讯作者：
and Murata Y.

Matrix Metalloprotease-9 Inhibition Improves Amyloid β-Mediated Cognitive Impairment and Neurotoxicity in Mice