精神機能に及ぼす甲状腺ホルモン作用におけるRCAN/カルシニューリン系の役割

RCAN(钙调神经磷酸酶)2是由甲状腺激素(T3)诱导的，它控制钙调神经磷酸酶(CaN)的活性。CaN是一种丰富存在于中枢神经系统中的蛋白质磷酸酶，据报道，异常存在于学习和记忆中，或通过敲除和过度表达而产生的精神状态。由于甲状腺功能减退导致脑内RCAN_2表达减少，RCAN_2深入参与了脑功能的调节，我们推测甲状腺激素对脑功能的影响可能是通过对RCAN_2基因表达的调节来实现的。这项研究通过对先前建立的RCAN2基因工程小鼠(基因敲除小鼠)的形态和行为分析，研究了RCAN2蛋白在脑功能中的作用。RCAN2KO小鼠出生正常，与野生型相比未见异常。我们建立了一个与C57BL/6J品系同源的品系，进行了回交、杂交，并将10代回交到…对11只(N10或11)F2小鼠进行行为学分析。此外，我们还将小鼠存放在理研生物资源中心。在初生期、生长期和成年期，与野生型小鼠相比，形态变异的RCAN2KO小鼠在大脑中没有RCAN2KO小鼠，未来需要进行更详细的调查。作为测量RCAN2KO小鼠行为数量的结果，差异[优势/KO小鼠、野生型和杂交型小鼠的自发行为数量]不被接受。然而，KO小鼠的行为量在移动到新的环境(用于测量的新笼子)后立即减少。此外，我认为这种差异与其他行为测试(高架式迷宫测试、Y字符迷宫测试、强迫游泳测试、恐惧症条件学习测试)所衡量的情绪行为和学习能力是不同的。日本理研生物资源中心开展的“日本小鼠诊所”的分析正在进行中。较少

RCAN (regulators of calcineurin) 2 is induced with a thyroxin (T3) and it controls the activity of a calcineurin (CaN). CaN is a protein phosphatase which exists in a CNS at a rich, and it is reported that unusuallies arise in a learning and a memory, or a mentation by the knock out and overexpression. Since the expression of cerebral RCAN2 reduced due to the hypothyroidism, RCAN2 participated in the cerebral function deeply and we assumed that the effect to the cerebral function of a thyroxin was demonstrated through the regulation of gene expression of RCAN2. This study examined the role in the cerebral function of RCAN2 protein through the morphological and behavioral analyses of the previously established RCAN2 genetically engineered mouse (knock out mouse). A RCAN2 KO mice is born normally and the abnormality compared with a wild type was not observed. We established as a congenic strain to C57BL/6J line, which carried out the back, cross, and made the backcross generation's 10 to … More 11 (N10or11) F2 mice, and provided the behavioral analyze. Moreover, we deposited the mice to the Riken bioresource center. In a nascent, a growth period, and an adult, a morphological variation does not have a RCAN2KO mice in a brain compared with a wild type mice, and a future still more detailed investigation is called for. As a result of measuring the amount of behaviourals of a RCAN2 KO mice, the difference [superiority / the amount of spontaneous behaviourals of KO mice, a wild type mice, and a hetero type mice] was not accepted. However, the amount of behavioral of KO mice was decreasing immediately after moving to a novel situation (novel cage for a measurement). Furthermore, I considered the difference to be an out-of thing to the emotional behavior and learning ability that have been measured by other behavioral trials (an elevated type crux maze trial, Y character maze trial, a forced-swimming trial, phobia conditional learning tests). The analyze by the "Japan mouse clinic" which a Riken bioresource center carry out is under perform. Less