Physiological and pathological functions of Favine

我们寻找了具有在小鼠主动脉中特异表达的信号序列的新基因，将其中一个基因命名为Favine。Favine在腹主动脉和脂肪组织中大量表达，肥胖db/db小鼠脂肪组织中Favine的mRNA水平高于正常小鼠。本研究构建了在肝脏中高表达Favine的转基因小鼠，并对肝脏、脂肪组织和主动脉进行了基因芯片分析。在转基因小鼠的主动脉中，肌肉收缩相关基因的表达水平显著增加。此外，在人主动脉平滑肌细胞中，这些基因的表达水平也可以通过加入重组Favine条件培养液来诱导。以上结果提示，法文对主动脉收缩有调节作用。为了检测人血清中Favine的水平，我们建立了抗体和ELISA法检测系统。在人血清中检测到Favine蛋白，其水平随饲养条件的变化而变化。

We searched for novel gene with signal sequences that are specifically expressed in murine aorta, designated one gene as Favine. Favine was expressed abundantly in the aorta and adipose tissues, and its mRNA levels were higher in adipose tissues of obese db/ db mice than control mice.In this study, we generated transgenic mouse overexpressing Favine in liver, and microarray analysis were performed in liver, adipose tissue, and aorta. In aorta of transgenic mouse, expression levels of genes associating muscle contraction were significantly increased. Moreover, in human aortic smooth muscle cells, expression levels of these genes were also induced by addition of recombinant Favine conditioned media. These results indicated that Favine should regulate aortic smooth muscle contraction. To measure Favine levels in human serum, we generated antiobody and ELISA assay system. Favine protein was detected in human serum, and its level changed depending on feeding conditions.