Physiological and pathological functions of Favine

Favine的生理和病理功能

• 批准号: 21591178
• 负责人: FUKUHARA Atsunori
• 金额: $ 3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591178/
• 关键词: 生活習慣病; ホルモン; Favine; Z5

We searched for novel gene with signal sequences that are specifically expressed in murine aorta, designated one gene as Favine. Favine was expressed abundantly in the aorta and adipose tissues, and its mRNA levels were higher in adipose tissues of obese db/ db mice than control mice.In this study, we generated transgenic mouse overexpressing Favine in liver, and microarray analysis were performed in liver, adipose tissue, and aorta. In aorta of transgenic mouse, expression levels of genes associating muscle contraction were significantly increased. Moreover, in human aortic smooth muscle cells, expression levels of these genes were also induced by addition of recombinant Favine conditioned media. These results indicated that Favine should regulate aortic smooth muscle contraction. To measure Favine levels in human serum, we generated antiobody and ELISA assay system. Favine protein was detected in human serum, and its level changed depending on feeding conditions.

我们寻找了一个在小鼠主动脉中特异表达的具有信号序列的新基因，命名为Favine。Favine在主动脉和脂肪组织中大量表达，肥胖db/ db小鼠脂肪组织中Favine的mRNA水平高于对照小鼠，本研究构建了肝脏中Favine过表达的转基因小鼠，并对肝脏、脂肪组织和主动脉进行了基因芯片分析。在转基因小鼠主动脉中，与肌肉收缩相关的基因的表达水平显著增加。此外，在人主动脉平滑肌细胞中，这些基因的表达水平也通过添加重组Favine条件培养基诱导。这些结果表明，Favine应该调节主动脉平滑肌收缩。为了测量人血清中的Favine水平，我们制备了抗体和ELISA测定系统。在人血清中检测到Favine蛋白，其水平根据饲养条件而变化。

项目成果

血管および脂肪組織由来新規分泌因子Favineの機能解析

Favine（一种源自血管和脂肪组织的新型分泌因子）的功能分析

• 发表时间: 2014
• 作者: 小林祥子;下村伊一郎
• 通讯作者: 下村伊一郎

Identification of a new secretory factor, CCDC3/Favine, in adipocytes and endothelial cells

• DOI: 10.1016/j.bbrc.2009.12.142
• 发表时间: 2010-01-29
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kobayashi, Sachiko;Fukuhara, Atsunori;Shimomura, Iichiro
• 通讯作者: Shimomura, Iichiro

キーストンシンポジア

基石研讨会

• 发表时间: 2010
• 作者: 小林祥子;福原淳範;田口貴史;大月道夫;下村伊一郎
• 通讯作者: 下村伊一郎

血管および脂肪組織由来新規分泌因子の同定

鉴定源自血管和脂肪组织的新型分泌因子

• 发表时间: 2010
• 作者: 小林祥子;福原淳範;松田守弘;大月道夫;下村伊一郎
• 通讯作者: 下村伊一郎