Mechanism of systemic lupus erythematosus accompanied with the aberrant TCR zeta mRNA 3'UTR

TCR zeta mRNA 3UTR异常引起系统性红斑狼疮的机制

• 批准号: 21591268
• 负责人: TSUZAKA Kensei
• 金额: $ 3.33万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591268/
• 关键词: TCR zeta; SLE; knockout mouse; 3'UTR; 全身性エリテマトーデス; TCR; シンデカン; EXTL2; ノックアウトマウス; TCRζ鎖; 3' UTR; RNA結合タンパク

To consider whether 3'UTR CS1 region prescribing stability of T-cell receptor zeta chain (TCR zeta) mRNA is associated with TCR zeta chain expression control, We made arm which is lack of CS1 and built a targeting vector. We introduced this targeting vector into an embryonic stem cell successively and made a recombinant embryonic stem cell. Furthermore, we injected it by the injection method and performed the manufacturing of chimera mouse. We crossbred this chimera mouse and wild-type mouse, and an F1 mouse was obtained. However, we tried the mating with the Cre mouse, but did not work well to remove pgk-neo cassette sandwiched in loxP incorporated in the genome of the F1 mouse.

为了研究T细胞受体zeta链（TCR zeta）mRNA的3 ′ UTR CS 1区是否与TCR zeta链的表达调控有关，我们制备了缺失CS 1的臂，构建了靶向载体。我们成功地将该靶向载体导入胚胎干细胞中，制备了重组胚胎干细胞。并通过注射法将其注射到小鼠体内，进行了嵌合体小鼠的制备。我们将该嵌合体小鼠与野生型小鼠杂交，获得了F1小鼠。然而，我们尝试了与Cre小鼠交配，但没有很好地去除夹在掺入F1小鼠基因组中的loxP中的pgk-neo盒。

项目成果

Single Center Prospective Study of Tacrolimus Efficacy and Safety in Treatment of Rheumatoid Arthritis

他克莫司治疗类风湿关节炎疗效和安全性的单中心前瞻性研究

• 发表时间: 2009
• 期刊: Rheum. Int 29(4)
• 作者: Suzuki K;Kameda H;Amano K;Nagasawa H;Takei H;Sekiguchi N;Nishi E;Ogawa H;Tsuzaka K;Takeuchi T
• 通讯作者: Takeuchi T

Plasma short-talin is a new rheumatoid arthritis monitoring biomarker independent of the inflammatory markers.

血浆短踝蛋白是一种独立于炎症标记物的新型类风湿性关节炎监测生物标记物。

• 发表时间: 2013
• 作者: Tsuzaka K;Takao M;Nishida J
• 通讯作者: Nishida J

Talin is cleaved and expressed as a short form predominantly in patients with rheumatoid arthritis

Talin 主要在类风湿性关节炎患者中被裂解并以短形式表达

• 发表时间: 2012
• 作者: Tsuzaka K;Takao Masako;Shinozaki N;Nishida J
• 通讯作者: Nishida J

IgG4-related systemic disease manifesting as Mikulicz disease with subsequent secondary portal hypertension and remarkable IgG4-linked IL-4 elevation

IgG4 相关全身性疾病，表现为 Mikulicz 病，随后继发性门脉高压和 IgG4 相关 IL-4 显着升高

• 发表时间: 2010
• 期刊: Rheumatology (in press)
• 作者: Suzuki K;Tamaru J;Okuyama A;Kameda H;Amano K;Nagasawa H;Nish E;Yoshimoto K;Setoyama Y;Kaneko K;Osada H;Honda N;Sasaki Y;Itoyama S;Tsuzaka K;Takeuchi T
• 通讯作者: Takeuchi T

IgG4関連自己免疫性疾患(形質細胞浸潤)

IgG4相关自身免疫性疾病（浆细胞浸润）

• 发表时间: 2009
• 期刊: 別刷日本臨床呼吸器症候群
• 作者: 井上明日香;松本功;住田孝之;Kida I;津坂憲政
• 通讯作者: 津坂憲政