Mechanism of systemic lupus erythematosus accompanied with the aberrant TCR zeta mRNA 3'UTR

为了研究t细胞受体zeta链（TCR zeta） mRNA稳定性的3'UTR CS1区是否与TCR zeta链表达控制有关，我们制作了缺乏CS1的手臂，构建了靶向载体。我们将该靶向载体先后导入胚胎干细胞，制备了重组胚胎干细胞。进一步采用注射法进行注射，并进行嵌合体小鼠的制备。我们将该嵌合体小鼠与野生型小鼠进行杂交，获得了一只F1小鼠。然而，我们尝试了与Cre小鼠的交配，但没有很好地去除F1小鼠基因组中合并的loxP中夹着的pgk-neo磁带。

To consider whether 3'UTR CS1 region prescribing stability of T-cell receptor zeta chain (TCR zeta) mRNA is associated with TCR zeta chain expression control, We made arm which is lack of CS1 and built a targeting vector. We introduced this targeting vector into an embryonic stem cell successively and made a recombinant embryonic stem cell. Furthermore, we injected it by the injection method and performed the manufacturing of chimera mouse. We crossbred this chimera mouse and wild-type mouse, and an F1 mouse was obtained. However, we tried the mating with the Cre mouse, but did not work well to remove pgk-neo cassette sandwiched in loxP incorporated in the genome of the F1 mouse.