Identification of target proteins of anti-endothelial cell antibodies in different racial groups with vasculitis by proteomics

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We attempted to identify candidate target proteins for anti-endothelial cell antibodies(AECA) comprehensively by a proteomic approach. Proteomic analyses showed significant different candidate target proteins between Japanese patients and American patients with large vessel vasculitis. More than 85% of Japanese patients with Takayasu arteritis(Tak) were found to have autoantibodies to Prx2 and PPIA, target proteins of AECA, respectively, whereas less than 15% of American patients with Tak were found to have them. Next, candidate target proteins for AECA were detected in American patients with giant cell arteritis(GCA) by two-dimensional electrophoresis-Western blotting. As a result, 9 endothelium-specific proteins and 22 patient-specific proteins were identified in GCA patients. 15 endothelium-specific spots and 4 patient-specific spots were detected in American patients with Tak. This work demonstrated that it is important to assess target proteins of AECA in different racial groups with vasculitis.