Identification of target proteins of anti-endothelial cell antibodies in different racial groups with vasculitis by proteomics

蛋白质组学鉴定不同种族血管炎抗内皮细胞抗体靶蛋白

• 批准号: 21591273
• 负责人: KARASAWA Rie
• 金额: $ 2.25万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591273/
• 关键词: 膠原病; 医化学; 血管炎; 抗血管内皮細胞抗体(AECA); プロテオミクス; 国際情報交換; アメリカ(北米)

We attempted to identify candidate target proteins for anti-endothelial cell antibodies(AECA) comprehensively by a proteomic approach. Proteomic analyses showed significant different candidate target proteins between Japanese patients and American patients with large vessel vasculitis. More than 85% of Japanese patients with Takayasu arteritis(Tak) were found to have autoantibodies to Prx2 and PPIA, target proteins of AECA, respectively, whereas less than 15% of American patients with Tak were found to have them. Next, candidate target proteins for AECA were detected in American patients with giant cell arteritis(GCA) by two-dimensional electrophoresis-Western blotting. As a result, 9 endothelium-specific proteins and 22 patient-specific proteins were identified in GCA patients. 15 endothelium-specific spots and 4 patient-specific spots were detected in American patients with Tak. This work demonstrated that it is important to assess target proteins of AECA in different racial groups with vasculitis.

我们试图通过蛋白质组学方法全面鉴定抗内皮细胞抗体（AECA）的候选靶蛋白。蛋白质组学分析显示，日本大血管炎患者和美国大血管炎患者的候选靶蛋白存在显著差异。超过85%的日本Takayasu动脉炎（Tak）患者分别检测到AECA靶蛋白Prx2和PPIA的自身抗体，而美国Tak患者检测到Prx2和PPIA抗体的比例不到15%。接下来，通过二维电泳- western blotting检测美国巨细胞动脉炎（GCA）患者中AECA的候选靶蛋白。结果，在GCA患者中鉴定出9种内皮特异性蛋白和22种患者特异性蛋白。在美国Tak患者中检测到15个内皮特异性斑点和4个患者特异性斑点。这项工作表明，在不同种族的血管炎患者中评估AECA的靶蛋白是重要的。

项目成果

血管炎患者における疾患特異的マーカーの検出

血管炎患者疾病特异性标志物的检测

• 发表时间: 2010
• 期刊: 腎と透析
• 作者: 唐澤里江;遊道和雄
• 通讯作者: 遊道和雄

抗血管内皮細胞抗体

抗血管内皮细胞抗体

• 发表时间: 2010
• 期刊: 日本臨床
• 作者: 唐澤里江;尾崎承一
• 通讯作者: 尾崎承一

Useful biomarkers for Takayasu's arteritis

高安动脉炎的有用生物标志物

• 发表时间: 2012
• 作者: Karasawa R;他4名
• 通讯作者: 他4名

Novel autoantigen for anti-endothelial cell antibody(AECA) identified by proteomics in vasculitis

蛋白质组学鉴定血管炎中抗内皮细胞抗体（AECA）的新型自身抗原

• 发表时间: 2010
• 作者: Karasawa R;Kato T;Ozaki S;Yudoh K
• 通讯作者: Yudoh K

The role of the autoantibodis to peroxiredoxin2, autoantigen for anti-endothelial cell antibody(AECA), in pathogenesis ofsystemic vasculitis

过氧化物酶2自身抗体、抗内皮细胞抗体（AECA）自身抗原在系统性血管炎发病机制中的作用

• 发表时间: 2010
• 作者: Karasawa R;Kato T;Ozaki S and Yudoh K
• 通讯作者: Ozaki S and Yudoh K