Analysis of membrane form of EGF family growth factors in the skin carcinogenesis using tissue-specific transgenic mice

为了阐明HB-EGF在皮肤癌发生中的作用，我建立了角质形成细胞特异性HB-EGF缺陷小鼠和角质形成细胞特异性四环素诱导型HB-EGF转基因小鼠，并进行了化学致癌和紫外线致癌研究。与对照组相比，HB-EGF转基因小鼠在早期阶段就发现了乳头状瘤和鳞癌。相反，在HB-EGF缺陷小鼠中几乎没有肿瘤生成。综上所述，HB-EGF在体内的过表达在皮肤肿瘤的形成机制中起重要作用。

In order to clarify the role of HB-EGF in skin carcinogenesis, I produced the keratinocyte-specific HB-EGF deficient mouse and the keratinocyte-specific tetracycline-inducible HB-EGF transgenic mouse, and then chemical and ultraviolet induced carcinogenesis studies were performed. Papilloma and squamous carcinoma were detected at an early stage in HB-EGF transgenic mice compared to control. Conversely, there was little tumor generating in HB-EGF deficient mice. Taking together, overexpression of HB-EGF in vivo plays important roles in the pathogenesis of skin tumor formation.