Study on mechanism of dermatitis due to epidermal barrier disruption : analysis of model mouse with epidermis devoid of ceramide

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Here, we generated Sptlc2 targeted mice under control of the keratin 5 promoter(referred to as K5-SPT-KO mice), by which their keratinocytes were devoid of SPT. These mice demonstrated lower water-holding capacity and barrier dysfunction. From 2 weeks of age, they developed skin inflammation, showing psoriasis-like histopathologic changes. K5-SPT-KO mice showed increased numbers ofγδ-T cells that produced IL-17(γδ-17) in the skin lesion and lymph nodes and most of them also produced IL-22, similar to Th17 cells. In vivo administration of anti-IL-12/ 23p40 antibody ameliorated the skin lesions and reducedγδ-17 cells in K5-SPT-KO mice. Therefore, we conclude that ceramide deficiency in the epidermis results in the development of psoriasis-like lesions.