Analysis of the effect of visible light on photoaged skin focusing on DNA repair

分析可见光对光老化皮肤的影响，重点关注DNA修复

• 批准号: 21591475
• 负责人: MORIWAKI Shinichi
• 金额: $ 2.91万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591475/
• 关键词: アンチエイジング; 光線治療; DNA修復; 発光ダイオード(LED); 紫外線; 宿主細胞回復; 酸化的DNA損傷; 紫外線性DNA損傷; メラニン; 酸化ストレス; DNA損傷; IPL; LED(発光ダイオード)

Visible light(VL) such as intense pulsed light(IPL) and light emitting diodes(LED) have been recently introduced to the painless, nonabrasive phototherapy to reverse photoaging skin such as wrinkles and lentigines. However the precise basic mechanisms on which VL irradiation can modulate these aged skin changes has not yet been proven. Here, we examined the effect of LED on cell proliferation, production of hyaluronic acid, DNA repair ability and melanogenesis by use of the human primary cultured fibroblasts and the 3-D skin-like tissue structure. A rate of cell proliferation and production of hyaluronic acid from the cells were significantly increased after red and blue LED irradiation. In addition, red, yellow and blue LED could stimulate the ability to repair oxidative DNA damage including 8-oxo-G of the cells assessed by host cell reactivation of the reporter plasmid. Melanogenesis was inhibited on the human 3-D skin-like tissue exposed to red or blue LED without no histologically damage of the skin. These findings suggest that LED light can be useful for skin rejuvenation through mechanisms of activating dermal skin fibroblast, increasing DNA repair ability to oxidative DNA damage and suppressing melanogenesis.

可见光（VL），如强脉冲光（IPL）和发光二极管（LED）最近已被引入到无痛，非研磨性光疗，以逆转光老化皮肤，如皱纹和雀斑。然而，VL照射可以调节这些老化皮肤变化的确切基本机制尚未得到证实。本研究以人皮肤成纤维细胞为研究对象，通过构建三维皮肤样组织结构，研究了LED对细胞增殖、透明质酸生成、DNA修复能力和黑素生成的影响。在红色和蓝色LED照射后，细胞增殖速率和来自细胞的透明质酸的产生显著增加。此外，红色、黄色和蓝色LED可以刺激修复氧化DNA损伤的能力，包括通过宿主细胞再激活报告质粒评估的细胞的8-oxo-G。红色或蓝色LED照射的人三维皮肤样组织黑素生成受到抑制，而皮肤组织学上无损伤。这些发现表明，LED光可以通过激活真皮皮肤成纤维细胞、增加DNA对氧化DNA损伤的修复能力和抑制黑色素生成的机制用于皮肤再生。

项目成果

正常ヒト皮膚3次元モデルを用いた赤色,青色LED(Light emitting diode)の色素沈着抑制効果の検討

使用正常人体皮肤的 3D 模型检查红色和蓝色 LED（发光二极管）的色素沉着抑制效果

• 发表时间: 2010
• 期刊: 日本美容皮膚科学会雑誌
• 作者: 森脇真一;小谷麻由美;櫟原維華;藤田晃人;岡崎茂俊
• 通讯作者: 岡崎茂俊

Identification of autoantibodies for α- and γ-enolase in serum from a patient with melanoma

黑色素瘤患者血清中 α- 和 γ-烯醇酶自身抗体的鉴定

• 期刊: Jpn Clin Med
• 作者: Hiura Y.Moriwaki S;et al
• 通讯作者: et al

Effects of red and blue light emitting diode (LED) on the cultured human skin

红光和蓝光发光二极管 (LED) 对培养人体皮肤的影响

• 发表时间: 2011
• 作者: Moriwaki S;Ichihara Y;Kotani M;Fujita;池田光徳;Moriwaki S
• 通讯作者: Moriwaki S

Experience of laboratory diagnosis of XP

XP实验室诊断体会

• 发表时间: 2010
• 作者: Song N;Boku S;Nakagawa S;Kato A;Toda H;Takamura N;Omiya Y;Kitaichi Y;Inoue T;Koyama T;Teraishi M;Moriwaki S
• 通讯作者: Moriwaki S

Ten Years of Clinical and Laboratory Work on Xeroderma Pigmentosum

色素性干皮病的十年临床和实验室工作

• 发表时间: 2010
• 期刊: Bulletin of the Osaka Medical College 56
• 作者: 斎藤三郎;秋山暢丈;Moriwaki S
• 通讯作者: Moriwaki S