Establishment of a new evaluation method of post-transplant immune tolerance by donor-specific regulatory T cell analysis

我们使用改进的ELISpot方法进行分析，以监测供者特异性同种异体反应性T细胞。肾移植后1年内同种异体反应性T细胞数无明显变化。我们还分析了排斥反应与外周血中Treg细胞数量的关系。一旦移植后Foxp3的表达降低，移植后一年恢复到移植前的水平。超过一年后不会发生急性排斥反应，因为Treg，而不是克隆性缺失和无能，可能是由于免疫调节。

We performed analysis by using the improved ELISpot assay for monitoring the donor specific allo reactive T cells. The numbers of allo reactive T cells were not altered in one year after kidney transplantation. We also analyzed about the relationship between rejection and Treg cells number in the peripheral blood. Once the expression of Foxp3 is lowered after transplantation, one year after the transplant was recovered up to the value before transplantation. Acute rejection does not occur more than one year after, because Treg, rather than clonal deletion and anergy, may be due to immune regulation.