The role of TRPV2 channel trafficking in cancer cell adhesion and motility

TRPV2 通道运输在癌细胞粘附和运动中的作用

• 批准号: 21591627
• 负责人: NAGASAWA Masahiro
• 金额: $ 2.91万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591627/
• 关键词: カルシウムチャネル; 癌細胞; 細胞運動; TRP; がん; カルシウム

TRPV2 channel was expressed in various cancer cells. In the present study, human fibrosarcoma cell lines was selected as a model system. Stimulation by EGF induced translocation of TRPV2 to the plasma membrane by a phosphatidylinositol (PI) 3-kinase-dependent mechanism. Moreover, an application of EGF caused a sustained increase of cytoplasmic calcium. Knock down of TRPV2 or TRP inhibitors blocked the changes in free calcium concentration. TRPV2 was localized at the filopodia or lamellipodia in the cells. TRPV2 also colocalized with focal adhesion structures, such as β-integrin, paxiliin, and vinculin. To further analyze these localization of TRPV2, vinculin conformation sensor FRET probe or vinculin tension sensor FRET probe was used. The vinculin conformation changes and calcium changes were observed around the front of the migrating cells. TRPV2 trafficking is related with the recycling of the plasma membrane.

TRPV2通道在多种癌细胞中表达。在本研究中，选择人纤维肉瘤细胞系作为模型系统。 EGF 刺激通过磷脂酰肌醇 (PI) 3 激酶依赖性机制诱导 TRPV2 易位至质膜。此外，EGF的应用导致细胞质钙的持续增加。 TRPV2 或 TRP 抑制剂的敲低阻止了游离钙浓度的变化。 TRPV2 定位于细胞中的丝状伪足或片状伪足。 TRPV2 还与粘着斑结构共定位，例如 β-整合素、桩蛋白和纽蛋白。为了进一步分析 TRPV2 的这些定位，使用了纽蛋白构象传感器 FRET 探针或纽蛋白张力传感器 FRET 探针。在迁移细胞前端周围观察到纽蛋白构象变化和钙变化。 TRPV2 运输与质膜的回收有关。

项目成果

Regulation of calcium-permeable TRPV2 channel by insulin in pancreatic beta-cells.

• DOI: 10.2337/db08-0862
• 发表时间: 2009-01
• 期刊: DIABETES
• 影响因子: 7.7
• 作者: Hisanaga, Etsuko;Nagasawa, Masahiro;Ueki, Kobjiro;Kulkarni, Rohit N.;Mori, Masatomo;Kojima, Itaru
• 通讯作者: Kojima, Itaru

Translocation of calcium-permeable TRPV2 channel to the podosome: Its role in the regulation of podosome assembly

• DOI: 10.1016/j.ceca.2011.12.012
• 发表时间: 2012-02-01
• 期刊: CELL CALCIUM
• 影响因子: 4
• 作者: Nagasawa, Masahiro;Kojima, Itaru
• 通讯作者: Kojima, Itaru

細胞接着・細胞運動とTRPV2機能制御

细胞粘附/细胞运动和TRPV2功能调节

• 发表时间: 2010
• 作者: 長澤雅裕;小島至
• 通讯作者: 小島至

Sweet taste receptor expressed in pancreatic beta-cells activates the calcium and cyclic AMP signaling systems and stimulates insulin secretion.

• DOI: 10.1371/journal.pone.0005106
• 发表时间: 2009
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Nakagawa Y;Nagasawa M;Yamada S;Hara A;Mogami H;Nikolaev VO;Lohse MJ;Shigemura N;Ninomiya Y;Kojima I
• 通讯作者: Kojima I

Microtubule Disruption with BAPTA and Dimethyl BAPTA by a Calcium Chelation-Independent Mechanism in 3T3-L1 Adipocytes

• DOI: 10.1507/endocrj.k08e-321
• 发表时间: 2009-04-01
• 期刊: ENDOCRINE JOURNAL
• 影响因子: 2
• 作者: Furuta, Ai;Tanaka, Marie;Shibata, Hiroshi
• 通讯作者: Shibata, Hiroshi