Analysis on factors relating metastasis in human gastric cancer cell line focusing on the metastasis patterns

为了阐明肿瘤的转移机制，我们在裸鼠身上建立了高度淋巴转移、肝脏转移和腹膜播散的模型，并对这些细胞转移的相关因素进行了表征。我们利用cDNA大阵列技术研究了肿瘤转移相关差异表达基因的生物学特性和mRNA水平。我们还进行了转移抑制物质的可用性以及转移潜能与血管生成因子的关系。迄今为止，内皮素在胃癌细胞系中的表达水平可能与肝转移有关，VEGF-C在胃癌细胞系中的表达水平可能与淋巴转移有关。骨桥蛋白也可能参与胰腺癌细胞系的肝转移。

To elucidate the mechanisms of cancer metastasis, we established highly lymphatic, liver metastatic and peritoneal disseminated models in nude mice, and then characterized several factors related to metastasis in these cells. We investigated several biological properties and mRNA levels of differentially-expressed genes involved in cancer metastasis by cDNA macroarray. We also conducted the availability of metastasis inhibition substances and the relation between metastatic potential and angiogenesis factorts. To date, Endothelin expression level in gastric cancer cell line might contribute to the liver metastasis and VEGF-C to the lymphatic metastasis. Osteopontin might also contribute to the liver metastasis in pancreatic cancer cell line.