A novel approach to treat acute respiratory failure by combination of type 2 alveolar epithelial regeneration and alveolar macrophage inhibition

结合2型肺泡上皮再生和肺泡巨噬细胞抑制治疗急性呼吸衰竭的新方法

• 批准号: 21591810
• 负责人: TSUCHIDA Masanori
• 金额: $ 3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591810/
• 关键词: 肺障害; 気道上皮再生; サイトカイン; 線維化; 培養細胞; 肺胞上皮; 遺伝子導入; HGF

In vitro cell culture experiment demonstrated that airway epithelial cell growth was improved by adding cytokines in the culture medium. Erectroporation also improved efficacy of gene introduction into culture cells of airway epithelium. Gene expression was observed in the alveolar epithelium by intratracheal administration of naked gene. However, lung injury due to pressure to epithelium was observed.

体外细胞培养实验表明，通过在培养基中添加细胞因子来改善气道上皮细胞的生长。勃起还提高了基因引入气道上皮培养细胞的功效。通过气管内给药裸基因在肺泡上皮中观察到基因表达。但是，观察到由于上皮压力而导致的肺损伤。