A novel approach to treat acute respiratory failure by combination of type 2 alveolar epithelial regeneration and alveolar macrophage inhibition

体外细胞培养实验表明，在培养液中加入细胞因子可促进呼吸道上皮细胞的生长。基因导入培养的呼吸道上皮细胞的效率也有所提高。通过气管内注射裸基因，可观察到肺泡上皮细胞基因的表达。但观察到上皮受压所致的肺损伤。

In vitro cell culture experiment demonstrated that airway epithelial cell growth was improved by adding cytokines in the culture medium. Erectroporation also improved efficacy of gene introduction into culture cells of airway epithelium. Gene expression was observed in the alveolar epithelium by intratracheal administration of naked gene. However, lung injury due to pressure to epithelium was observed.