Morphological and functional analyses of osteoclast-like cells using time-lapse imaging by video-enhanced microscopy

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We established the stable fluorescent condition by actin stained osteoclast-like cells and analyzed morphological changes of vacuole structures to visualize the morphological changes of intracellular structure and acid secretion mechanism in bone resorption process by osteoclast. As the bone resorption activity was growing, it was visualized that the intracellular vacuole structures morphologically changed and were eccentrically distributed to the bigger cells. After adding elcatonin, bone resorption agent, this activity of osteoclast-like cells changed. This distribution seems to be characteristic changes in osteoclast-like cells growing the bone resorption activity.