Morphological and functional analyses of osteoclast-like cells using time-lapse imaging by video-enhanced microscopy

使用视频增强显微镜延时成像对破骨细胞样细胞进行形态学和功能分析

• 批准号: 21591940
• 负责人: HOSHINO Hironobu
• 金额: $ 2.75万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591940/
• 关键词: 破骨細胞; 骨吸収; ビデオマイクロスコピー; アクチン; 顕微鏡

We established the stable fluorescent condition by actin stained osteoclast-like cells and analyzed morphological changes of vacuole structures to visualize the morphological changes of intracellular structure and acid secretion mechanism in bone resorption process by osteoclast. As the bone resorption activity was growing, it was visualized that the intracellular vacuole structures morphologically changed and were eccentrically distributed to the bigger cells. After adding elcatonin, bone resorption agent, this activity of osteoclast-like cells changed. This distribution seems to be characteristic changes in osteoclast-like cells growing the bone resorption activity.

通过肌动蛋白染色的破骨细胞样细胞建立稳定的荧光条件，并分析空泡结构的形态变化，以可视化破骨细胞在骨吸收过程中细胞内结构的形态变化和排酸机制。随骨吸收活性增加，可见细胞内空泡结构形态发生改变，偏心分布于较大的细胞内。加入骨吸收制剂--降钙素后，破骨细胞样细胞的这种活性发生了变化。这种分布似乎是破骨细胞样细胞生长成骨吸收活动的特征性变化。

项目成果

Quantitative evaluation of bone resorption activity of osteoclast-like cells by measuring calcium phosphate resorbing area using incubator-facilitated and video-enhanced microscopy

通过使用培养箱辅助和视频增强显微镜测量磷酸钙吸收面积来定量评估破骨细胞样细胞的骨吸收活性

• 发表时间: 2009
• 期刊: Microsc Res Tech
• 作者: Morimoto Y;Hoshino H;Sakurai T;Terakawa S;Nagano A
• 通讯作者: Nagano A