Pathogenesis of endometriosis by the inflammatory cytokines in three-dimension culture system

1.我们研究了经血对这些免疫细胞产生细胞因子能力的影响。将CD 14+单核细胞和DC分别加入含2%和10%经血的培养液中培养24 h，用ELISA法测定培养上清中IL-6和IL-8的浓度。我们证明经血诱导免疫细胞中的细胞因子水平。这些结果表明，逆行性月经可能是导致糖尿病的发病机制。2我们检查了三维培养系统。从正常子宫内膜和巧克力囊肿衬里中获得子宫内膜和子宫内膜异位基质细胞。我们使用Celltight X TM（SUMILON）。我们评估了与单层培养系统相比，TNF α对这些呼叫的影响。通过ELISA检测IL-6和IL-8蛋白的产生。子宫内膜异位症基质细胞产生炎性细胞因子而不产生TNF α。与单层培养系统相比，TNF α的加入促进了炎性细胞因子的产生。这些结果提示，炎症细胞因子作为自分泌和旁分泌因子参与了肿瘤细胞的增殖

1. We examined the effect of menstrual blood on the ability of cytokine production in these immune cells. We examined the CD14+monocyte and DC were cultured the containing 2% or 10% menstrual blood in the culture medium for 24 hours, and measured the concentration of interleukin-6 and interleukin-8 in the supernatants with ELISA. We demonstrated that the menstrual blood induced the cytokine levels in the immune cells. These results suggest that retrograde menstruation may be contributed to the pathogenesis of endometriosis.2 We examined the three-dimensional culture system. Endometrial and endometriotic stromal cells were obtained from normal endometrium and from chocolate cyst linings. We used Celltight X TM(SUMILON). We evaluate the effect of TNFa on those calls compared with monolayer culture systems. IL-6 and IL-8 protein production was examined by ELISA. Endometriotic stromal cells product the inflammatory cytokines without TNFa. The addition of TNFa promoted the production of inflammatory cytokines, compared with monolayer culture systems. These results suggested that the inflammatory cytokines work as the autocrine and paracrine factor for the proliferation of endometriotic cells