Analysis of immunosuppressive microenvironment associated with Epithelial-Mesenchymal Transition in uterine cancer.

与子宫癌上皮间质转化相关的免疫抑制微环境分析。

• 批准号: 21592144
• 负责人: FUJITA Tomonobu
• 金额: $ 2.91万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592144/
• 关键词: 癌; 子宮体癌; 子宮頚癌; EMT; 免疫抑制; 上皮間葉転換

To analyze immunosuppressive tumor microenvironment associated with EMT, novel cancer-associated genes MYEOV and HOXD9 were investigated. After the transfection of MYEOV siRNA into the endometrial cancer cell lines, migration index and invasion index were both significantly lower than that in the control group. mRNA expression of Snail1, Snail2, ZEB1 was significantly reduced, while fibronectin was significantly increased. Silencing of HOXD9 inhibits cell proliferation in 4 of 4 cervical cancer cells, but there were no EMT related gene that was commonly inhibited nor stimulated in 4 cervical cell lines.

为了分析与EMT相关的免疫抑制肿瘤微环境，研究了新的癌症相关基因MYEOV和HOXD9。MYEOV siRNA转染子宫内膜癌细胞系后，迁移指数和侵袭指数均显著低于对照组。Snail1、Snail2、ZEB1 mRNA表达量显著降低，纤维连接蛋白显著升高。在4个宫颈癌细胞系中，HOXD9的沉默抑制了4个宫颈癌细胞的细胞增殖，但在4个宫颈癌细胞系中没有常见的抑制或刺激EMT相关基因。

项目成果

RNA Helicase Encoded by Melanoma Differentiation-Associated Gene 5 Is a Major Autoantigen in Patients With Clinically Amyopathic Dermatomyositis Arthritis Rheum

黑色素瘤分化相关基因 5 编码的 RNA 解旋酶是临床无肌病性皮肌炎关节炎大黄患者的主要自身抗原

• 发表时间: 2009
• 作者: Sato S;Hoshino K;Satoh T;Fujita T;Kawakami Y;Fujita T;Kuwana M
• 通讯作者: Kuwana M

RNA Helicase Encoded by Melanoma Differentiation-Associated Gene 5 Is a Major Autoantigen in Patients With Clinically Amyopathic Dermatomyositis.

由黑色素瘤分化相关基因 5 编码的 RNA 解旋酶是临床无肌病性皮肌炎患者的主要自身抗原。

• 发表时间: 2009
• 期刊: Arthritis Rheum. 60(7)
• 作者: Sato S;et al.
• 通讯作者: et al.

Epithelial Mesenchymal Transition in Human Ocular Chronic Graft-Versus-Host Disease

• DOI: 10.2353/ajpath.2009.090318
• 发表时间: 2009-12-01
• 期刊: AMERICAN JOURNAL OF PATHOLOGY
• 影响因子: 6
• 作者: Ogawa, Yoko;Shimmura, Shigeto;Tsubota, Kazuo
• 通讯作者: Tsubota, Kazuo

Blockade of activated NF-kB in cancer and immune cells mayeliminate immunosuppressive state in human ovarian cancer

阻断癌症和免疫细胞中活化的 NF-kB 可能消除人类卵巢癌的免疫抑制状态

• 发表时间: 2010
• 作者: Hiroshi Nishio;Tomonori Yaguchi;Tomonobu Fujita;Kazuo Umezawa;Takashi Iwata;Takuma Fujii;Hidetoshi Sumimoto;Daisuke Aoki;Yutaka Kawakami
• 通讯作者: Yutaka Kawakami

卵巣癌細胞株におけるPDL1の発現とErk1/2カスケードとの関連

卵巢癌细胞株中PDL1表达与Erk1/2级联反应的关系

• 发表时间: 2009
• 作者: 岩田卓;藤田知信;守井賢二;宮崎潤一郎;西尾浩;谷口智憲;藤井琢磨;青木大輔;河上裕
• 通讯作者: 河上裕