Mitochondrial DNA deletion or depletion on the acquisition of drug tolerance in head and neck cancer

通过剂量递增暴露于顺铂或多西他赛来选择耐化疗剂的头颈癌细胞系。提取单个耐药细胞的线粒体DNA（mtDNA），并对全长线粒体基因组进行PCR扩增，以检查单个细胞的mtDNA。我们可以在耐药细胞中检测到缺失的mtDNA。否则，我们不能建立从新鲜冷冻头颈癌组织的激光显微切割获得的单细胞中可重复扩增的mtDNA。

Chemotherapeutic agent-resistant head and neck cancer cell lines were selected by a dose-escalating exposure to cisplatin or docetaxel. Mitochondrial DNA(mtDNA) from individual drug-resistant cells was extracted and subjected to PCR amplification of full-length mitochondrial genomes to examine mt DNA from single cells. We could detect deleted form of mt DNA in drug-resistant cells. Otherwise, we could not establish mt DNA reproducible amplification from single cells derived by laser micro dissection of fresh frozen head and neck cancer tissues.