Intraoperative diagnostic system for the metastasis of sentinel lymph node using the genetic technique

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One-step nucleic acid amplification(OSNA) is a new automated rapid system to detect lymph node metastasis using the genetic technique. The purpose of this study was to develop the OSNA method to identify lymph node metastasis in head and neck cancer.Analysis of 22 cases of head neck squamous cell carcinoma was performed with OSNA method and with routine histological examination. The OSNA and histological results were identical. According to this study, OSNA method has the potential to diagnose cervical node metastasis in head and neck cancer.