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Basic research about the development of the allogeneic reconstituted skin equivalent which induces local immunotolerance

Basic research about the development of the allogeneic reconstituted skin equivalent which induces local immunotolerance
诱导局部免疫耐受的同种异体重建皮肤等同物开发的基础研究
批准号：
21592290
负责人：
MATSUMOTO Yoshitaka
金额：
$2.33万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
项目状态：
已结题
起止时间：
2009 至 2011
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592290/
关键词：
組織培養移植学同種皮膚移植免疫寛容サイトカイン再構築皮膚3次元培養組織工学不死化株HPV E7

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英文摘要
Basic research was performed with inbred rat strain to develope the allogeneic reconstituted skin equivalent inducing local immunotolerance.Keratinocyte and fibroblast was derived from rat skin, and the keratinocyte was immortalized(AK-E7). The reconstituted skin equivalent, using AK-E7 with fibroblast, showed nearly normal histologic epidermal structure. The cultured supernatant of the fibroblast transfected with IL-10 cDNA(AFB-IL10) inhibited mixed lymphocyte reaction. As the reconstituted skin equivalent using AK-E7 and AFB-IL10 was grafted into autograft model, its partial take was recognized histologically.
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