Analysis for genetic alteration of oral precancerous lesions

口腔癌前病变的基因改变分析

• 批准号: 21592337
• 负责人: SEMBA Ichiro
• 金额: $ 2.91万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592337/
• 关键词: 口腔癌前癌病変; 4NQO誘発ラット舌癌モデル; 遺伝子変異; 遺伝子発現異常RT-PCR; 免疫組織化学; 病理組織形態; DNA損傷修復; 遺伝子発現異常; RT-PCR

In this study, we successfully established field-cancerization animal model of tongue carcinoma. The model provides well compatibility of macroscopic and microscopic findings and analysis for chronological and site specific changes of the precancerous lesion. In this model, the precancerous lesion shows diffuse epithelial dysplasia and the grade of histological atypia was increased during the carcinogen administration period, although interruption of the administration of the carcinogen brought decrease the atypia but further advanced lesions such as focal protrude lesion and invasive carcinoma.There is high level expression of genes related with double strands DNA damage repair in the precancerous lesions, and it suggests that different properties from in the invasive carcinoma.

本研究成功地建立了舌癌野化动物模型。该模型提供了良好的兼容性的宏观和微观的研究结果和分析的时间和部位的具体变化的癌前病变。在该模型中，癌前病变表现为弥漫性上皮异型增生，且在致癌剂给药期间组织学分级增加，虽然中断致癌剂给药导致上皮异型增生减少，但进一步发展为局灶性突出病变和浸润性癌。在癌前病变中存在与双链DNA损伤修复相关的基因的高水平表达，这表明了与浸润性癌不同的性质。

项目成果

Pathological analysis of precancerous lesion in 4NQO-induced rat tongue carcinogenesis experimental model

4NQO致大鼠舌癌实验模型癌前病变的病理分析

• 发表时间: 2011
• 作者: Y. Oyazato;M. Hirano;K. Shima;I. Semba
• 通讯作者: I. Semba

4NQO誘発ラット舌前癌病変モデル確立と前癌病変におけるDNA損傷修復機構の解析

4NQO诱导大鼠舌癌前病变模型的建立及癌前病变DNA损伤修复机制分析

• 发表时间: 2012
• 作者: 親里嘉貴;平野真人;嶋香織;仙波伊知郎
• 通讯作者: 仙波伊知郎

Oral Precancerous lesions and diagnosis

口腔癌前病变及诊断

• 发表时间: 2009
• 作者: Y. Oyazato;M. Hirano;K. Shima;I. Semba;仙波伊知郎
• 通讯作者: 仙波伊知郎