The mechanism of signal transduction of perlecan in the tooth enamel organ morphogenesis

基底膜蛋白聚糖在牙釉质器官形态发生中的信号转导机制

• 批准号: 21592321
• 负责人: IDA Hiroko
• 金额: $ 3万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592321/
• 关键词: 発生・分化; シグナル伝達; 歯胚エナメル器; パールカン

Perlecan, a heparan sulfate proteoglycan, plays an important role in cellular growth, differentiation, adhesion and motility by its interaction with growth factors and cytokines. During odontogenesis, perlecan started to be localized in the central area of the epithelial tooth bud, and with the maturation of the enamel organ, it accumulated in the intercellular spaces of the stellate reticulum.To understand the role of perlecan in enamel organ morphogenesis, we analyzed a keratin 5-perlecan transgenic mice that over-express perlecan in epithelial cells, and examined their tooth germs. The overexpression of perlecan in the enamel organ resulted in irregular morphology of teeth, suggesting that the expression of perlecan regulates growth factor signaling in a stage-dependent manner during each step of the interaction between ameloblast-lineage cells and mesenchymal cells. The time schedule of the intraepithelial expression of perlecan seems to be controlled critically in the process of odontogenesis.

Perlecan是一种硫酸乙酰肝素蛋白多糖，通过与生长因子和细胞因子的相互作用，在细胞的生长、分化、黏附和运动中发挥重要作用。在牙胚发育过程中，Perlecan开始定位于上皮性牙蕾的中央区域，随着釉质器官的成熟，它聚集在星状网状结构的细胞间隙中。为了了解Perlecan在釉质器官形态发生中的作用，我们分析了一只在上皮细胞中过表达Perlecan的角蛋白5-Perlecan转基因小鼠，并检测了它们的牙胚。在成釉细胞系细胞与间充质细胞相互作用的各个阶段，Perlecan蛋白的过度表达导致牙齿形态不规则，提示Perlecan蛋白的表达以阶段依赖的方式调节生长因子信号转导。在牙齿形成过程中，Perlecan在上皮内表达的时间进程似乎受到了严格的控制。

项目成果

Differential expression profiles between α-dystroglycan and integrin β1 in ameloblastoma : two possible perlecan signaling pathways for cellular growth and differentiation

成釉细胞瘤中 α-肌营养不良聚糖和整合素 β1 之间的差异表达谱：细胞生长和分化的两种可能的基底膜蛋白信号传导途径

• 发表时间: 2011
• 期刊: Histopathology
• 影响因子: 6.4
• 作者: Ida-Yonemochi H;Ahsan MS;Saku T
• 通讯作者: Saku T

Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

• DOI: 10.1007/s00418-010-0727-5
• 发表时间: 2010-09-01
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Ishikawa, Yuko;Ida-Yonemochi, Hiroko;Ohshima, Hayato
• 通讯作者: Ohshima, Hayato

Matrix metalloproteinase 7 and perlecan in oral epithelial dysplasia and carcinoma in situ : an aid for histopathologic recognition of their cell proliferation centers

基质金属蛋白酶7和基底膜蛋白聚糖在口腔上皮发育不良和原位癌中的作用：有助于组织病理学识别其细胞增殖中心

• DOI: 10.1111/j.1600-0714.2009.00750.x
• 发表时间: 2009
• 期刊: Journal of Oral Pathology & Medicine
• 影响因子: 3.3
• 作者: Tilakaratne WM;Kobayashi T;Ida-Yonemochi H;Swelam W;Yamazaki M;Mikami T;Alvarado CG;Ahsan MS;Maruyama S;Cheng J;Saku T.
• 通讯作者: Saku T.

マウス歯胚発育過程におけるATBF-1の局在

ATBF-1 在小鼠牙胚发育过程中的定位

• 发表时间: 2010
• 作者: Ishihara;K.;et al;Toshiyuki Kawakami;依田浩子
• 通讯作者: 依田浩子

Enzymatically synthesized glycogen has osteogenic potential in vitro and in vivo

酶法合成的糖原在体外和体内具有成骨潜力

• 发表时间: 2011
• 作者: Ida-Yonemochi H;Takata H;Tanaka M;Nakagawa E;Kenmotsu S;Ohshima H
• 通讯作者: Ohshima H