Fundamental Evaluation of taste dysfunction in the aging society as a New Rapid Diagnostic Method using Carbonic Anhydrase VI in Parotid Saliva

据了解，70%以上的味觉功能障碍患者都是缺锌所致。碳酸酐酶（CA） VI，存在于人类唾液中的金属锌蛋白，与味觉功能障碍有关。因此，我们开发了一种新的快速简便的诊断方法，使用免疫层析法作为替代血液检查的现场测试。首先，对血清抗体的反应性进行评估，并与对照进行比较，并用胶体金进行检测。在PBS（-）和阳性对照（合成肽）样品之间观察到显色差异。此外，它是密度依赖性的，并且在连续稀释的腮腺唾液中。结果提示，利用该抗体建立的免疫层析法可定量测定CA VI，为锌缺乏症味觉功能障碍的诊断提供参考。

It is known that more than 70% of patients with taste dysfunction have zinc deficiency. Carbonic anhydrase(CA) VI, zinc metallo-protein found in human saliva and is involved in taste dysfunction. Therefore, we developed a new quick and simple method of diagnosis using an immunochromatography assay to be used as an on-site test in substitution for a blood test. First, reactivity with a serum antibody was evaluated and compared to a control and detected with colloidal gold. A difference in color development was observed between samples of PBS(-) and the positive control(synthetic peptide). Furthermore, it was density dependent, and in serially diluted parotid gland saliva. The results suggest that an immunochromatography assay using this novel antibody can be used to quantitatively measure CA VI, which may be useful in the diagnosis of taste dysfunction caused by zinc deficiency.