Study on novel tissue regenerative therapy using bone marrow-derived mesenchymal stem cells and FGF/Phosphophoryn/Collagen complex

利用骨髓间充质干细胞和 FGF/磷酸化/胶原复合物进行新型组织再生疗法的研究

• 批准号: 21592630
• 负责人: FUJII Takeo
• 金额: $ 2.91万
• 依托单位: Matsumoto Dental University (2011)Health Sciences University of Hokkaido (2009-2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592630/
• 关键词: 歯周外科学; 再生治療; 三次元培養系; 歯髄間葉系幹細胞; ホスホホリン; FGF; 再生医療; 歯周組織再生; 骨髄間葉系幹細胞

With the goal to establish new tissue regenerative therapy for extensive regeneration of destroyed periodontal tissues accompanying vertical or horizontal bone defects due to severe periodontitis, The aim of this study was to evaluate the kinetics of bone marrow-derived mesenchymal stem cells cultured in a FGF. collagen complex as a regenerative scaffold in vitro. Bone marrow-derived mesenchymal stem cells showed good growth in the collagen gel culture. The effect of FGF in promoting cell growth is exhibited at the early stage of culture, and the ALP activity has already passed the peak when assayed at 14 days of culture at FGF concentrations of 10 ng/ml. The ALP activity showed in the presence of phosphophoryn in a dose-dependent manner at 14 days of culture. A marked difference in calcium content was not observed. The present study sheds light on the development of an optimal biomaterial that forms the basis of calcified hard tissue formation, suggesting the feasibility of applying the 3-D collagen gel cell culture system for the construction of a scaffold that controls the amount of regenerating tissue.

目的是建立新的组织再生治疗广泛再生的破坏牙周组织伴随垂直或水平骨缺损，由于严重的牙周炎，本研究的目的是评估骨髓间充质干细胞培养在FGF的动力学。胶原复合物作为体外再生支架。骨髓间充质干细胞在胶原凝胶中生长良好。FGF促进细胞生长的作用在培养早期就表现出来，在10 ng/ml的FGF浓度下培养14天时，ALP活性已过峰值。培养14 d时，磷酸化蛋白的存在对ALP活性的影响呈剂量依赖性。未观察到钙含量的显著差异。本研究揭示了形成钙化硬组织形成基础的最佳生物材料的发展，表明应用3-D胶原凝胶细胞培养系统构建控制再生组织量的支架的可行性。

项目成果

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients

FGF-2在牙周炎患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T.Fujii;Y.Terada;M.Akamatsu;M.Watanuki
• 通讯作者: M.Watanuki

歯周炎罹患歯に対する FGF-2投与の長期的効果および安全性の検討

检查 FGF-2 给药对受牙周炎影响的牙齿的长期影响和安全性

• 发表时间: 2012
• 期刊: 日歯周誌
• 作者: 北村正博;古市保志;藤井健男;川浪雅光;國松和司;島内英俊;山田 了;小方頼昌;和泉雄一;伊藤公一;中川種昭;新井 高;山崎和久;吉江弘正;野口俊英;渋谷俊昭;高柴正悟;栗原英見;永田俊彦;横田 誠;前田勝正;廣藤卓雄;坂上竜資;原 宜興;野口和行;小笠原健文;村上伸也
• 通讯作者: 村上伸也

Periodontal Tissue Regeneration by the Application of FGF-2 in Patients

FGF-2在患者中应用的牙周组织再生

• 发表时间: 2010
• 作者: T. Fujii;Y. Terada;M. Akamatsu;M. Watanuki
• 通讯作者: M. Watanuki

Periodontal Tissue Regeneration by the Application of FGF-2 in Periodontitis Patients.

FGF-2 在牙周炎患者中的应用实现牙周组织再生。

• 发表时间: 2009
• 期刊: J Clin Periodontol 36
• 作者: T.Fujii;Y.Terada;M.Akamatsu;M.Watanuki.
• 通讯作者: M.Watanuki.

Murakami FGF-2 stimulates periodontal regeneration : results of multicenter randomized clinical trial

Murakami FGF-2 刺激牙周再生：多中心随机临床试验结果

• 发表时间: 2011
• 期刊: J Dent Res
• 影响因子: 7.6
• 作者: Kitamura M;Akamatsu M;Machigashira M;Hara Y;Sakagami R;Hirofuji T;Hamachi T;Maeda K;Yokota M;Kido J;Nagata T;Kurihara H;Takashiba S;Sibutani T;Fukuda M;Noguchi T;Yamazaki K;Yoshie H;Ioroi K;Arai T;Nakagawa T;Ito K;Oda S;Izumi Y;Oga
• 通讯作者: Oga