Analysis of glial-progenitor-neural networks and role of nitric oxide to maintain neuropathic pain

巢蛋白是一种在鼻咽癌细胞中表达的VI类中间丝。为了研究神经元和神经胶质细胞是否在原位增殖或从含有神经前体细胞的特定区域迁移，我们对Nestin启动子GFP转基因小鼠进行了L5脊神经切断，以缩小靶点至神经前体细胞。提示周围神经损伤后，神经前体细胞的增殖和向神经元和星形胶质细胞的分化可能参与了周围神经损伤后晚期神经病理性疼痛的维持。

Nestin is a class VI intermediate filament expressed in NPCs. To study whether neurons and glia proliferate in situ or migrate from specific regions harboring neural progenitor cells, we applied L5 spinal nerve transection to nestin-promoter GFPtransgenic mice to narrow the target to neural progenitor cells. It was suggested that proliferation of neural progenitor cells and their differentiation into neurons and astrocytes occur in the dorsal horn in response to peripheral nerve injury and that they may contribute to the maintenance of neuropathic pain at the late phase after peripheral nerve injury.