Ligand recognition mechanism of the ecdysone hormone receptor for the molecular design of a new pesticide

蜕皮激素受体的配体识别机制用于新型农药的分子设计

• 批准号: 21603011
• 负责人: TADA Toshiji
• 金额: $ 2.58万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21603011/
• 关键词: 蛋白質結晶学; 構造生物化学; 分子認識; 昆虫; 構造活性相関; 蛋白質; 分析科学; 生体機能利用; 環境対応

Insects grow by repeatedly molting. The aim of this work is to elucidate the molecular mechanism of the molting hormone receptor by analyzing its crystal structure. The receptor composed of two proteins, EcR and USP, was coexpressed in E coli. Crystallization trials were performed using the purified receptor proteins in order to determine the three dimensional structure by X-ray crystallography. Small crystals were obtained, but the structure determination could not be achieved in a period due to difficulty of its crystal growth. However, it has been found that the receptor can be crystallized without the molting hormone.

昆虫通过反复蜕皮而生长。本工作的目的是通过分析蜕皮激素受体的晶体结构来阐明蜕皮激素受体的分子机制。该受体由两种蛋白质EcR和USP组成，在大肠杆菌中共表达。使用纯化的受体蛋白进行结晶试验，以通过X射线晶体学确定三维结构。获得了小晶体，但由于晶体生长困难，结构测定不能在一段时间内实现。然而，已经发现受体可以在没有蜕皮激素的情况下结晶。

项目成果

キイロショウジョウバエEcR/USPの結晶化

黑腹果蝇 EcR/USP 的结晶

• 发表时间: 2009
• 作者: 蒲池沙織;中江摂;原田俊幸;小倉岳彦;中川好秋;多田俊治
• 通讯作者: 多田俊治