Ligand recognition mechanism of the ecdysone hormone receptor for the molecular design of a new pesticide

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Insects grow by repeatedly molting. The aim of this work is to elucidate the molecular mechanism of the molting hormone receptor by analyzing its crystal structure. The receptor composed of two proteins, EcR and USP, was coexpressed in E coli. Crystallization trials were performed using the purified receptor proteins in order to determine the three dimensional structure by X-ray crystallography. Small crystals were obtained, but the structure determination could not be achieved in a period due to difficulty of its crystal growth. However, it has been found that the receptor can be crystallized without the molting hormone.