Study on development of in situ tip-enhanced Raman spectroscopy for characterizing the surface and inside of a living stem cells.

将大鼠成体骨髓间充质干细胞（r-MSCs）培养在不含细胞因子的蜂窝膜和FI-PEARL膜上。异常的细胞粘附和分裂发现，r-MSCs完全不扩散，沿着培养保持半球形的原始形状，直接在半球形的细胞体中分裂。在PE-PEARL膜上的增殖能力高于在市售聚苯乙烯培养板上的增殖能力。这些膜使MSCs无需常规胰蛋白酶处理，只需用水冲洗即可轻松恢复。本研究表明，这两种膜都是间充质干细胞有前途的支架，使间充质干细胞的无生物风险传代培养成为可能。我们获得的技术数据有助于构建基于尖端增强Rama散射的复杂技术，用于表征活间充质干细胞的表面和内部。

Mesenchymal stem cells from rat adult bone marrow(r-MSCs) were cultured on Honeycomb films and FI-PEARL films without cytkines for cell growth. The anomalous cellular adhesion and division were found in such a way that r-MSCs did not spread at all keeping semispherical original shape along culture and divided directly in the semispherical cell bodies. The proliferative ability on PE-PEARL films was higher than that on a commercially available polystyrene culture plate. These films enabled easy recovery of MSCs by rinsing with water without the conventional trypsin treatment. The present study suggested that both films are promising scaffolds for mesenchymal stem cells which make the bio risk free subculture of MSCs possible. We obtained technical data useful for constructing a sophisticated technique based on tip-enhanced Rama scattering for characterizing the surface and inside of a living mesenchymal stem cell.