Study on development of in situ tip-enhanced Raman spectroscopy for characterizing the surface and inside of a living stem cells.

将成年大鼠骨髓间充质干细胞(r-MSCs)接种于不含细胞因子的蜂窝膜和透明质膜上进行细胞培养。细胞黏附和分裂异常，r-MSCs完全不扩散，沿培养方向保持半球形，直接在半球形胞体内分裂。在PE-珍珠膜上的增殖能力高于在市售聚苯乙烯培养板上的增殖能力。这些膜使MSCs能够在没有常规胰酶处理的情况下，通过水冲洗轻松恢复。本研究表明，这两种材料都是骨髓间充质干细胞的良好支架材料，为骨髓间充质干细胞的无生物风险传代提供了可能。我们获得了有用的技术数据，用于构建一种基于尖端增强拉玛散射的复杂技术，用于表征活的间充质干细胞的表面和内部。

Mesenchymal stem cells from rat adult bone marrow(r-MSCs) were cultured on Honeycomb films and FI-PEARL films without cytkines for cell growth. The anomalous cellular adhesion and division were found in such a way that r-MSCs did not spread at all keeping semispherical original shape along culture and divided directly in the semispherical cell bodies. The proliferative ability on PE-PEARL films was higher than that on a commercially available polystyrene culture plate. These films enabled easy recovery of MSCs by rinsing with water without the conventional trypsin treatment. The present study suggested that both films are promising scaffolds for mesenchymal stem cells which make the bio risk free subculture of MSCs possible. We obtained technical data useful for constructing a sophisticated technique based on tip-enhanced Rama scattering for characterizing the surface and inside of a living mesenchymal stem cell.