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食生活改善による概日リズムを介した癌の予防

通过改善饮食习惯，通过昼夜节律预防癌症

基本信息

批准号：
21650198
负责人：
平石さゆり
金额：
$ 2.05万
依托单位：
Kagawa Nutrition University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21650198/
关键词：
食生活癌概日リズム転移栄養学

项目摘要

末梢組織の概日リズムは食事に影響を受けることが知られている。食餌摂取時刻の変化によって概日リズムが乱れることが、癌の転移や進展を悪化させるかを検討した。1.癌転移実験 ICR雄性の野生型マウス、あるいは、概日リズム関わる時計遺伝子の一つであるclockの変異型ICR(Clock)マウスを各々明期に給餌させる群(昼給餌群)と、暗期に給餌させる群(夜給餌群)に分けて飼育繁殖用の飼料を与え、マウスの尾静脈から癌細胞(マウスメラノーマ)を投与した後の肺への転移と血漿中プラスミノーゲン活性化阻害因子(PAI-1)量を調べた。その結果、通常行動しない時刻に摂食させた野生型マウスの昼給餌群は、夜給餌群に比べて肺への転移癌結節数が多かった。また、Clockマウスの夜および昼給餌群は共に野生型マウスの夜給餌群より癌の転移が亢進したが、Clockマウスの夜給餌群と昼給餌群との間にはほとんど差がなかった。血漿中PAI-1濃度も野生型マウスの夜給餌群に比べ昼給餌群およびClockマウスで高値となったが、Clockマウスでは夜給餌群と昼給餌群との間にはほとんど差がなかった。PAI-1の発現には日内変動があるが、食餌時刻の変化によってこの概日リズムが崩れてPAI-1濃度が増加したことが考えられた。また、正常な概日リズムを刻まないClockマウスで癌の転移が亢進した。これらから、通常は行動しない時間帯に摂食させると、概日リズムが乱れ、その結果、癌の転移が亢進することが考えられた。2.発癌実験 ICR雄性マウスにDiethylnitrosamine(20mg/kg)を腹腔内投与して、7週齢から昼夜給餌群と昼給餌群に分けて飼育した。投与後経時的に採血し、血漿中のALT、AST活性を測定し、35週目で解剖して肝、脾臓および胸腺を摘出し、発癌の状態を観察した。その結果、腫瘍性結節の最大径・結節数、および血漿中ALT、AST活性ともに昼夜給餌群と昼給餌群に差はほとんどなかった。以上より、食事時刻の変化は概日リズムを撹乱させ、癌の転移に影響を与える可能性が示唆された。

The end of the organization is expected to receive information about the food market on the weekend. At the same time, the food supply should be changed at the same time. On the first day of the day, there will be chaos, cancer, cancer, and cancer. 1. Cancer transfer ICR male wild-type mice, wild-type mice, and almost daily wild-type ICR (Clock) mice were divided into two groups for breeding, breeding and breeding. The amount of activating inhibitory factor (PAI-1) in the blood of the lungs was significantly higher than that of the control. The results showed that the number of metastatic cancer in the lungs was much higher than that in the lungs, and the results showed that during the exercise, the wild type was fed to the population during the day, and the night was given to the population. During the night, the wild type of Clock was used to treat the population, and the wild type was used to increase the activity of cancer, while the Clock was used to increase the activity of cancer. The concentration of PAI-1 in the blood is higher than that of the wild type in the blood, the concentration of the wild type is higher than that of the Clock, and that of the Clock is higher than that of the wild type. The PAI-1 will be scheduled to have an emergency in the following days, such as the availability of food, the collapse of the weather, the increase of the PAI-1 schedule, and the training of the weather. The disease, the normal day, the day, day, During the normal exercise period, the food intake, the daily food consumption, the results of the test, the results of the test, and the results of the test, and the results of the test. two。 The ICR male mice were injected intraperitoneally with Diethylnitrosamine (20mg/kg), and the mice were fed day and night by day and night. The activity of ALT and AST in the blood, the liver and spleen were dissected and the thymus was removed and the cancer was examined at 35 years old. The results of the test, the number of the maximum diameter of the sexual results, the activity of ALT and AST in the blood were measured day and night, and the difference between the two groups was observed. The above information, the possibility of cancer transfer and the possibility of instigation are indicated by the above information, the daily schedule, the possibility of cancer transfer and the possibility of cancer.