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Effect of Vitamin D3 on Antimicrobial Peptides Expression in Keratinocyte.

维生素 D3 对角质形成细胞抗菌肽表达的影响。

基本信息

批准号：
21792093
负责人：
KURASHIGE Yoshihito
金额：
$ 2.16万
依托单位：
Health Sciences University of Hokkaido
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21792093/
关键词：
活性型ビタミンD3 抗細菌性ペプチドディフェンシン LL-37

项目摘要

The innate immune system of mammals provides a rapid response to repel assaults from numerous infectious agents including bacteria, viruses, fungi, and parasites. A major component of this system is a diver combination of cationic antimicrobial peptides that include the α- and β-defensins (hBDs), and cathelicidin (LL-37). hBDs and LL-37 belong to a group of antimicrobial peptides mainly produced by the epithelial cells of many organ including skin, lung, kidney, pancreas, urerus, eye, and oral epithelium. The oral epithelium consists of stratified keratinocytes with a variable degree of keratinization. The molecular components of cellular tight junctions, including claudins, occludin and ZO-1, contribute to homeostasis in oral epithelial keratinocytes. Many functions of keratinocytes are regulated by 1,25-dihydroxyvitamin D3 (1,25-D3), the active form of vitamin D, that includes inhibition of proliferation, stimulation of differentiation and promotion of innate and adaptive immunity. It is still unclear whether 1,25-D3 affects the expression of hBDs, LL-37, claudin, ocludin and ZO-1 in keratinoccytes. The keratinocytes incubated with 1,25-D3 induced upregulated expression of hBD-1, hBD-2, LL-37 and cla-4, downregulated expression of cla-1 in a dose- and time-dependent manner. Incubation with 1,25-D3 at any dose did not alter expression level of hBD-1, -3.

哺乳动物的先天免疫系统提供快速反应以击退来自包括细菌、病毒、真菌和寄生虫的许多感染因子的攻击。该系统的主要组分是阳离子抗微生物肽的不同组合，包括α-和β-防御素（hBD）和凯萨林菌素（LL-37）。hBD和LL-37属于一组主要由许多器官的上皮细胞产生的抗微生物肽，所述器官包括皮肤、肺、肾、胰腺、输尿管、眼和口腔上皮。口腔上皮由具有不同程度角化的分层角化细胞组成。细胞紧密连接的分子组成部分，包括claudins，occludin和ZO-1，有助于口腔上皮角质形成细胞的稳态。角质形成细胞的许多功能由维生素D的活性形式1，25-二羟基维生素D3（1，25-D3）调节，包括抑制增殖、刺激分化以及促进先天性和适应性免疫。目前尚不清楚1，25-D3是否影响角质形成细胞中hBD、LL-37、claudin、ocludin和ZO-1的表达。1，25-D3诱导角质形成细胞hBD-1、hBD-2、LL-37和cla-4表达上调，cla-1表达下调，呈剂量和时间依赖性。与任何剂量的1，25-D3孵育均未改变hBD-1、-3的表达水平。