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Platelet-activating factor-mediated regulation of angiogenesis in the chorionic villi and the decidua.

血小板激活因子介导的绒毛膜绒毛和蜕膜血管生成调节。

基本信息

批准号：
20591921
负责人：
NARAHARA Hisashi
金额：
$ 3万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591921/
关键词：
血小板活性化因子絨毛脱落膜着床血管新生

项目摘要

Platelet-activating factor(PAF), a potent lipid mediator, has been implicated in a number of reproductive processes, including implantation. PAF has been shown to be produced by human embryo, which appears to be a requisite for viability. However the precise role of embryo-derived PAF in implantation has yet to be established. To investigate the role of PAF in chorionic or decidual angiogenesis, we evaluated the production of various angiogenic factors or anti-angiogenic factors by trophoblast cells(TC) or decidual cells(DC). We measured the concentrations of three angiogenic factors in the culture media of TC and DC following stimulation with an analogue, carbamyl-PAF(C-PAF). This substrate is not metabolized. These three factors were vascular endothelial growth factor(VEGF), basic fibroblast growth factor(b-FGF) and angiogenin. Enzyme-linked immunosorbent assay was used. In addition, we evaluated the expression of thrombospondin(TSP)-1 protein in TC and DC after stimulation with C-PAF using western blot analysis. The levels of VEGF, b-FGF, and angiogenin in the culture media of the C-PAF-stimulated TC and DC rose as an increase in the amount of C-PAF added. These C-PAF-induced effects were abolished following pretreatment with PAF receptor antagonists, Y-24180 and CV2086. A PKC activator, TPA also stimulated these angiogenic factors, which was abolished by a PKC inhibitor, H-7. C-PAF had no effect on TSP-1 production in TC and DC. It is suggested that the PAF secreted by the decidual tissue and by the developing embryo stimulates via PKC the production of these angiogenic factors at the feto-maternal interface. The up-regulation of these factors in the local environment may be important in the angiogenesis during early stages of gestation.

血小板活化因子(PAF)是一种强有力的脂质介质，参与了包括着床在内的许多生殖过程。PAF已被证明是由人类胚胎产生的，这似乎是生存所必需的。然而，胚胎来源的PAF在着床中的确切作用尚未确定。为了探讨PAF在绒毛或蜕膜血管生成中的作用，我们评估了滋养层细胞(TC)或蜕膜细胞(DC)产生的各种血管生成因子或抗血管生成因子。用氨甲酰-PAF(C-PAF)刺激TC和DC培养上清液中三种血管生成因子的浓度。这种底物不会被代谢。这三种因子分别是血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(b-FGF)和血管生成素。采用酶联免疫吸附测定法。用免疫印迹法检测C-PAF刺激后TC和DC中TSP-1蛋白的表达。在C-PAF刺激的TC和DC中，随着C-PAF加入量的增加，培养上清液中血管内皮生长因子、b-成纤维细胞生长因子和血管生成素的水平升高。用PAF受体拮抗剂Y-24180和CV2086预先阻断C-PAF的上述效应。作为一种PKC激活剂，TPA也刺激这些血管生成因子，但这种刺激被PKC抑制剂H-7所消除。C-PAF对TC和DC中TSP-1的产生没有影响。提示蜕膜组织和发育中的胚胎分泌的PAF通过PKC刺激胎儿-母体界面血管生成因子的产生。这些因素在局部环境中的上调可能在妊娠早期的血管生成中起重要作用。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of heparin-binding EGF-like growth factor and amphiregulin on the MAP kinase-induced production of vascular endothelial growth factor by human granulosa cells

DOI：
10.3109/08977194.2011.607136
发表时间：
2011-12-01
期刊：
GROWTH FACTORS
影响因子：
1.8
作者：
Karakida, Sinya;Kawano, Yasushi;Narahara, Hisashi
通讯作者：
Narahara, Hisashi

Decidualization attenuates the contractility of endometriotic stromal cells

蜕膜化减弱子宫内膜异位基质细胞的收缩力

DOI：
发表时间：
2012
期刊：
影响因子：
0
作者：
Nasu K;Tsuno A;Yuge A;Kawano Y;Abe W;Kai K;Nishida M;Kawano Y;Narahara H
通讯作者：
Narahara H

Cadmium chloride induces the expression of metallothionein mRNA by endometrial stromal cells and amnion-derived(WISH) cells

氯化镉诱导子宫内膜基质细胞和羊膜来源（WISH）细胞表达金属硫蛋白 mRNA

DOI：
发表时间：
2011
期刊：
Gynecol Obstet Invest
影响因子：
0
作者：
Yasushi Kawano;Yuichi Furukawa;Yukie Kawano;Wakana Abe;Tomoko Hirakawa;Hisashi Narahara
通讯作者：
Hisashi Narahara

Erucylphosphocholine induces growth inhibition, cell cycle arrest, and apoptosis in human choriocarcinoma cells

芥酰磷酸胆碱诱导人绒毛膜癌细胞生长抑制、细胞周期停滞和细胞凋亡