Analysis of propagation manners of paramyxoviruses in neural cells.

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Both Measles and Nipah virus causes severe acute or late-onset encephalitis in human. In this study, we aimed to investigate the mechanisms of measles and nipah virus spread between neural cells. We established the AMCA (on-chip agarose microchamber array) system for primary neural cells, and elucidated the propagation manner of MV to neighboring cells. By using fluorescent labeled- viral protein, a new domain, which was important for interaction of NiV N and P protein, was identified. Further, we investigated NiV spread in African green monkey by using EGFP-expressing recombinant NiV. The results indicated that lethal infection to CNS occurred after systemic infection.