Study of radiation-induced cluster DNA damage- its structure and repair susceptibility

为了阐明簇状DNA损伤的诱导过程，我们利用生物化学和光谱技术研究了DNA分子的辐射效应。我们测定了不同的离子束照射引起的簇状DNA损伤的产率。使用计算机模拟方法对数据进行分析，以建立损伤诱导模型。此外，利用安装在高亮度同步辐射装置上的EPR装置，我们发现了一种参与DNA损伤诱导的新的自由基过程。这些成果已在若干专题讨论会或讲习班上作了介绍，并作为25篇同行评议论文发表。

In order to clarify the induction processes of clustered DNA damage, we have investigated the radiation effect on DNA molecules using both biochemical and spectroscopic techniques. We determined the yields of clustered DNA damage induced by various ion beam exposure. The data were analyzed using a computer simulation method to establish a damage induction model. Further we found a novel radical process involved in the DNA damage induction using an EPR apparatus installed in a high brilliance synchrotron radiation facility. These results have been presented in a number of symposiums or workshops, and also published as 25 peer review papers.