Large-scale differential display system with vertebrate-common degenerate oligonucleotide primers

为了研究环境污染物对多种生物基因表达的可能影响，我们基于退化寡核苷酸引物PCR（dopo -PCR）建立了一个大型差异显示系统，该系统包含了7种脊椎动物中常见的引物集，并具有详细的分子背景。从鱼类到灵长类，7种脊椎动物的转录本中均含有8个mer的反向重复基序，共有1万多个基序。其中，我们选择了275个共同基序，覆盖了这些物种中约30%的转录本，制作了7个脊椎动物共同DOP- pcr引物（common DOP引物）。该系统使我们能够在鸡和鸬鹚的胎儿肝脏中找到一些对2,3,7,8 -四氯二苯并-对二恶英（TCDD）有反应的基因。

To study possible impacts of environmental pollutants on gene expression in a variety of organisms, we developed a large-scale differential display system with primer sets that are common in 7 vertebrate species with detailed molecular background, based on degenerate oligonucleotide-primed PCR(DOP-PCR). Inverse repeat motif of 8 mer was found in most transcripts for 7 vertebrates examined from fish to primates and more than ten thousand motifs were commonly recognized in transcripts of these 7 species. Among these, we chose 275 common motifs that cover roughly 30% of transcripts throughout these species, making seven vertebrates-common DOP-PCR primers(common DOP primers). This system enabled us to find some genes responsive to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in fetal livers of chicken and common cormorant.