Large-scale differential display system with vertebrate-common degenerate oligonucleotide primers

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To study possible impacts of environmental pollutants on gene expression in a variety of organisms, we developed a large-scale differential display system with primer sets that are common in 7 vertebrate species with detailed molecular background, based on degenerate oligonucleotide-primed PCR(DOP-PCR). Inverse repeat motif of 8 mer was found in most transcripts for 7 vertebrates examined from fish to primates and more than ten thousand motifs were commonly recognized in transcripts of these 7 species. Among these, we chose 275 common motifs that cover roughly 30% of transcripts throughout these species, making seven vertebrates-common DOP-PCR primers(common DOP primers). This system enabled us to find some genes responsive to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in fetal livers of chicken and common cormorant.