Detection and analysis of DNA repair using chemically-synthesized DNA sensors

DNA总是受到外源性和内源性因素的损伤，但所有生物体都有损伤DNA的修复系统。我们打算开发通过荧光检测和分析DNA修复反应的传感器（或技术上称为探针），并合成用于碱基切除修复酶与氧化损伤DNA反应的荧光探针。使用这些探针，在体外分析了修复酶的底物特异性，并成功地可视化了细胞修复反应。

DNA is always damaged by exogenous and endogenous factors, but all living organisms have repair systems for damaged DNA. We intended to develop sensors(or technically known as probes) to detect and analyze DNA repair reactions by fluorescence, and synthesized fluorescent probes that would be used for the reactions of base-excision-repair enzymes with oxidatively-damaged DNA. Using these probes, the substrate specificities of the repair enzymes were analyzed in vitro, and the cellular repair reactions were successfully visualized.