Elucidation of the molecular mechanism of photosynthetic water oxidation by means of infrared spectroscopy

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We have investigated the molecular mechanism of photosynthetic water oxidation using light-induced Fourier transform infrared difference spectroscopy and revealed the number of protons released upon individual intermediate transitions, the efficiencies of transitions, the structures and the roles of amino-acids coupled to the Mn cluster, the effect of an inhibitor on water oxidation reactions, and the regulation mechanism by extrinsic proteins. We also studied proton-coupled electron transfer in the water oxidation reaction using time-resolved infrared spectroscopy and obtained experimental evidence that proton is released before the electron transfer in the S3→S0transition, in which molecular oxygen is evolved.