Molecular mechanism of cell polarity establishment and cell-face determination by RNA localization and local translation

基因表达的转录后调控在细胞生长、细胞分化、适应应激和细胞死亡等多种细胞过程中发挥着重要作用。在我们的研究中，我们的重点是了解rna结合蛋白在酵母和哺乳动物细胞中的转录后调控的分子机制和生理功能。我们发现rna结合蛋白Khd1和细胞质deadenylase Ccr4通过调节RhoGEF和RhoGAP的表达来调节酵母细胞壁完整性通路中Rho1的信号。我们还发现，rna结合蛋白Stau1通过调控编码Wnt通路中心介质的Dvl2 mRNA来协调肌肉发生。

Post-transcriptional regulation of gene expression has a significant role in various cellular processes such as cell growth, cell differentiation, adaptation to stress, and cell death. In our study, we are focusing on understanding the molecular mechanism and the physiological function of the post-transcriptional regulation by RNA-binding proteins using yeast and mammalian cells. We have revealed that RNA-binding protein Khd1 and cytoplasmic deadenylase Ccr4 modulate a signal from Rho1 in the yeast cell wall integrity pathway by regulating the expression of RhoGEF and RhoGAP. We have also revealed that RNA-binding protein Stau1 coordinates myogenesis through the regulation of Dvl2 mRNA encoding a central mediator of Wnt pathway.