Elucidation of molecular basis of insect resistance in mungbean using comparative genomics information

点击翻译按钮获取中文摘要

In order to elucidate the molecular basis of bruchid resistance derived from a wild accession of mungbean(Vigna radiata), we constructed a fine linkage map and a detailed physical map around the insect resistance locus(Br) using a large-scale segregating population and three mungbean BAC libraries. Recombinant lines and their progenies indicated that the two distinct regions in the same linkage group are responsible for the resistance. In addition, RNA-Seq analysis revealed one gene, which strongly expressed in developing seeds of the resistant mungbean lines, was identified from each region harboring the resistance gene(s). These two genes shared a high degree of homology, but the function of the genes as well as their homologous genes in other plant species has not been annotated yet.