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Functional analysis of spermidine for conferring abiotic stress tolerance

亚精胺赋予非生物胁迫耐受性的功能分析

基本信息

批准号：
21380028
负责人：
MORIGUCHI Takaya
金额：
$ 6.32万
依托单位：
National Agriculture and Food Research Organization
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380028/
关键词：
ポリアミン環境ストレススペルミジン組換え体セイヨウナシ

项目摘要

Transgenic European pear lines with reduced spermidine(spd) synthase(SPDS) expression showed severe effect on phenotypes compared with the wild-type(WT) under salt or cadmium stress. However, repressed phenotype was much alleviated by application of Spd. Application of Spd to WT can confer stress tolerance, while application of SPDS inhibitor to SPDS-overexpressing pear showed reduction of stress tolerance. We also showed the evidence for the importance of Spd in stress tolerance by influencing antioxidant system. Moreover, several genes including dehydrin and peroxidase whose expression levels were significantly different in SPDS-overexpressing line and WT, were successfully isolated by differential display and cDNA microarray.

与野生型（WT）相比，盐或镉胁迫下，转基因欧洲梨株系与减少亚精胺（spd）合酶（SPDS）的表达表现出严重的影响表型。然而，抑制表型大大减轻应用Spd。Spd对WT的施用可以赋予胁迫耐受性，而SPDS抑制剂对SPDS过表达的梨的施用显示胁迫耐受性降低。我们还通过影响抗氧化系统，证明了Spd在植物抗逆性中的重要性。利用差异显示技术和cDNA微阵列技术，成功分离到了SPDS过表达株和野生型中表达量差异显著的几个基因，包括Bispherin和过氧化物酶。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Antisense inhibition of a spermidine synthase gene highlights the role of polyamines for stress alleviation in pear shoots subjected to salinity and cadmium

DOI：
10.1016/j.envexpbot.2011.03.001
发表时间：
2011-09
期刊：
Environmental and Experimental Botany
影响因子：
5.7
作者：
X. Wen;Y. Ban;H. Inoue;N. Matsuda;M. Kita;T. Moriguchi
通讯作者：
X. Wen;Y. Ban;H. Inoue;N. Matsuda;M. Kita;T. Moriguchi

Identification of differentially expressed genes potentially related to stress tolerance in a transgenic line of European pear over-expressing an apple spermidine synthase gene

过表达苹果亚精胺合酶基因的欧洲梨转基因品系中鉴定可能与胁迫耐受性相关的差异表达基因

DOI：
发表时间：
2011
期刊：
J. Hort. Sci. Biotechnol
影响因子：
0
作者：
Xiao-Peng Wen;Yusuke Ban;Xiao-Ming Pang and Takaya Moriguchi
通讯作者：
Xiao-Ming Pang and Takaya Moriguchi

Antisense inhibition of a spermidine synthase gene highlights a role of polyamines for stress alleviation in pear shoots subjected to salinity and cadmium

亚精胺合成酶基因的反义抑制凸显了多胺在缓解盐度和镉胁迫下梨芽应激中的作用

DOI：
发表时间：
2011
期刊：
Environ. Exp. Bot
影响因子：
0
作者：
Xiao Peng Wen;Yusuke Ban;Horomichi Inoue;Narumi Matsuda;Masayuki Kita and Takaya Moriguchi
通讯作者：
Masayuki Kita and Takaya Moriguchi

Identification of differentially-expressed genes potentially related to stress tolerance in a transgenic line of European pear over-expressing an apple spermidine synthase gene (MdSPDS1)

过表达苹果亚精胺合酶基因 (MdSPDS1) 的欧洲梨转基因品系中鉴定可能与胁迫耐受性相关的差异表达基因