Research on a disturbance mechanism of endocrine system of host by an insect virus.

昆虫病毒扰乱宿主内分泌系统机制的研究

• 批准号: 21380038
• 负责人: TAKATSUKA Jun
• 金额: $ 12.48万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380038/
• 关键词: 昆虫; ウイルス; 内分泌; ウィルス

Whole genome sequences of entomopoxviruses revealed the viruses had an important gene for manipulation of the host endocrine. Using cultured cell that was found to support an entomopoxvirus replication in present research, knock-out mutant lacking this gene function was constructed. Juvenile hormone(JH) titer of the hosts infected with wild type virus was maintained to be high, resulting in host's death in larval stage. On the other hand, JH titer of the hosts infected with the gene function-defective virus was similar to that of healthy hosts, which allowed infected hosts to develop toward pupation. These findings indicate that entomopoxviruses have a gene that disturbs JH titer to manipulate hosts development.

昆虫痘病毒的全基因组序列揭示了该病毒具有操纵宿主内分泌的重要基因。利用本研究发现的支持虫痘病毒复制的培养细胞，构建了缺乏该基因功能的敲除突变体。感染野生型病毒后，寄主的幼体激素（JH）滴度维持在较高水平，导致寄主在幼虫期死亡。另一方面，基因功能缺陷病毒感染宿主的JH滴度与健康宿主相似，这使得感染宿主向化蛹方向发展。这些发现表明，虫痘病毒具有一种干扰JH滴度以操纵宿主发育的基因。

项目成果

アワヨトウ昆虫ポックスウイルスMythimna separate entomopoxvirus (Myse EV)の培養細胞への感染性

秋粘虫昆虫痘病毒Mythimna分离昆虫痘病毒(Myse EV)对培养细胞的感染性

• 发表时间: 2011
• 作者: Sanada-Morimura S.;et al.;高務淳
• 通讯作者: 高務淳