Structural biology for elucidation of lysine biosynthesis and its regulatory mechanisms
Structural biology for elucidation of lysine biosynthesis and its regulatory mechanisms
批准号:
21380057
负责人:
NISHIYAMA Makoto
金额:
$12.23万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
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英文摘要
There are two different pathways in lysine biosynthesis. In this study, we have succeeded in elucidation of regulatory mechanisms of enzymes, each of which has a key role in each biosynthetic pathway. In the second half of lysine biosynthetic pathway throughα-aminoadipate, we discovered that a protein named LysW is not only used for modification of the amino group of the substrate, but also served as a carrier protein for biosynthetic enzymes.
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Enhancement of latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution
通过定向进化增强混杂高异柠檬酸脱氢酶的潜在 3-异丙基苹果酸脱氢酶活性
DOI:
--
发表时间:
2010
期刊:
Biochem J
影响因子:
4.1
作者:
[Y. Suzuki, K. Asada, J. Miyazaki, T. Tomita, T. Kuzuyama, and M. Nishiyama]
通讯作者:
and M. Nishiyama
Enhancement of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus
增强底物识别并深入了解嗜热栖热菌高柠檬酸合酶的反馈抑制
DOI:
--
发表时间:
2010
期刊:
Journal Biological Chemistry
影响因子:
--
作者:
[T.Okada, ら]
通讯作者:
ら
Mechanism for multiple-substrates recognition of α-aminoadipate aminotransferase from Thermus thermophilus
嗜热栖热菌α-氨基己二酸转氨酶的多底物识别机制
DOI:
--
发表时间:
2009
期刊:
Proteins-Structure Function and Bioinformatics 75
影响因子:
--
作者:
[Tomita, T., et al.]
通讯作者:
et al.
グルタミン酸脱水素酵素の新規活性調節機構の発見
谷氨酸脱氢酶活性调控新机制的发现
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[西山真, 富田武郎]
通讯作者:
富田武郎
旧くて新しいアミノ酸生合成研究
新旧氨基酸生物合成研究
DOI:
--
发表时间:
2009
期刊:
影响因子:
--
作者:
[西山真]
通讯作者:
西山真
共 34 条
Structure-function relationship of novel lysine biosynthetic enzymes and analysis of molecular evolution of the pathway
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批准号:16380056
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$10.05万
-
财政年份:2004
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负责人:NISHIYAMA Makoto
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依托单位:
Analysis of novel lysine biosynthesis in Thermus and its regulation
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批准号:13660079
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.62万
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财政年份:2001
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负责人:NISHIYAMA Makoto
-
依托单位:
Analysis of mechanisms of regulation of aspartate kinase and lysine biosyntheseis in Thermus thermophilus
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批准号:11660072
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.43万
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财政年份:1999
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负责人:NISHIYAMA Makoto
-
依托单位:
Studies on electron-transferring mechanism in an nitrite-reducing system containing Cu-proteins
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批准号:09660075
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.43万
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财政年份:1997
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负责人:NISHIYAMA Makoto
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依托单位:
海外基金