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Structural biology for elucidation of lysine biosynthesis and its regulatory mechanisms

Structural biology for elucidation of lysine biosynthesis and its regulatory mechanisms
阐明赖氨酸生物合成及其调控机制的结构生物学
批准号:
21380057
负责人:
NISHIYAMA Makoto
金额:
$12.23万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011

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中文摘要
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英文摘要
There are two different pathways in lysine biosynthesis. In this study, we have succeeded in elucidation of regulatory mechanisms of enzymes, each of which has a key role in each biosynthetic pathway. In the second half of lysine biosynthetic pathway throughα-aminoadipate, we discovered that a protein named LysW is not only used for modification of the amino group of the substrate, but also served as a carrier protein for biosynthetic enzymes.
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Enhancement of latent 3-isopropylmalate dehydrogenase activity of promiscuous homoisocitrate dehydrogenase by directed evolution
通过定向进化增强混杂高异柠檬酸脱氢酶的潜在 3-异丙基苹果酸脱氢酶活性
DOI: --
发表时间: 2010
期刊: Biochem J
影响因子: 4.1
作者: [Y. Suzuki, K. Asada, J. Miyazaki, T. Tomita, T. Kuzuyama, and M. Nishiyama]
通讯作者: and M. Nishiyama
Enhancement of substrate recognition and insight into feedback inhibition of homocitrate synthase from Thermus thermophilus
增强底物识别并深入了解嗜热栖热菌高柠檬酸合酶的反馈抑制
DOI: --
发表时间: 2010
期刊: Journal Biological Chemistry
影响因子: --
作者: [T.Okada, ら]
通讯作者:
DOI: --
发表时间: 2009
期刊: Proteins-Structure Function and Bioinformatics 75
影响因子: --
作者: [Tomita, T., et al.]
通讯作者: et al.
グルタミン酸脱水素酵素の新規活性調節機構の発見
谷氨酸脱氢酶活性调控新机制的发现
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [西山真, 富田武郎]
通讯作者: 富田武郎
34
    Structure-function relationship of novel lysine biosynthetic enzymes and analysis of molecular evolution of the pathway
    • 批准号:
      16380056
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $10.05万
    • 财政年份:
      2004
    • 负责人:
      NISHIYAMA Makoto
    • 依托单位:
    Analysis of novel lysine biosynthesis in Thermus and its regulation
    • 批准号:
      13660079
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.62万
    • 财政年份:
      2001
    • 负责人:
      NISHIYAMA Makoto
    • 依托单位:
    Analysis of mechanisms of regulation of aspartate kinase and lysine biosyntheseis in Thermus thermophilus
    • 批准号:
      11660072
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.43万
    • 财政年份:
      1999
    • 负责人:
      NISHIYAMA Makoto
    • 依托单位:
    Studies on electron-transferring mechanism in an nitrite-reducing system containing Cu-proteins
    • 批准号:
      09660075
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.43万
    • 财政年份:
      1997
    • 负责人:
      NISHIYAMA Makoto
    • 依托单位:
    海外基金