Development of techniques for diagnosis and prevention of fish disease by the analysis of T cell function in fish immune response

通过分析鱼类免疫反应中的 T 细胞功能开发诊断和预防鱼类疾病的技术

• 批准号: 21380126
• 负责人: NAKANISHI Teruyuki
• 金额: $ 11.23万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380126/
• 关键词: 魚類; T細胞; 細胞性免疫; ギンブナ; CD4; CD8; IFNγ; パーフォリン; グランザイム; モノクローナル抗体

In this project, we showed a) tissue distribution of T cell subset, and the characteristics of CD4^+helper T cells using monoclonal antibodies in ginbuna, b) killing mechanisms and differentiation and activation of CD8^+ cytotoxic T lymphocyte, c) primary importance of cell-mediated immunity in the defense against cell-associate bacteria, d) establishment of basic tools for the induction of Th1 response in fish, e) identification of immune genes and analysis of immune mechanisms in rainbow trout, flounder and yellowtail which enable the studies on cell-mediated immunity in important fishes in aquaculture.

In this project, we showed a) tissue distribution of T cell subset, and the characteristics of CD4^+helper T cells using monoclonal antibodies in ginbuna, b) killing mechanisms and differentiation and activation of CD8^+ cytotoxic T lymphocyte, c) primary importance of cell-mediated immunity in the defense against cell-associate bacteria, d) establishment of basic tools for the induction of Th1在鱼类中的反应，e）鉴定免疫基因和彩虹鳟鱼，比目鱼和黄尾尾的免疫机制的分析，这使水产养殖中重要鱼类的细胞介导的免疫力进行了研究。

项目成果

コイ末梢血白血球の培養におけるBリンパ球の増殖

鲤鱼外周血白细胞培养中B淋巴细胞的增殖

• 发表时间: 2010
• 作者: 仁井田祥光;片倉文彦;山口卓哉;森友忠昭;中西照幸
• 通讯作者: 中西照幸

Development of the single-cell colonyforming assay system : characterization of carp(Cyprinus carpio) kidney hematopoietic progenitor cells

单细胞集落形成测定系统的开发：鲤鱼（Cyprinus carpio）肾造血祖细胞的表征

• 发表时间: 2009
• 作者: Katakura F.;M. Yoshida;T. Yamaguchi;T. Moritomo;and T. Nakanishi
• 通讯作者: and T. Nakanishi

Expression analysis of immune genes in ginbuna, Carassius auratus langsdorfii upon vaccination with Aeromonas hydrophila

嗜水气单胞菌接种后银鱼、鲫鱼免疫基因的表达分析

• 发表时间: 2009
• 作者: Ohtani M.;H. Toda;T. Moritomo and T. Nakanishi
• 通讯作者: T. Moritomo and T. Nakanishi

ウイルス感染で誘導されたギンブナ細胞傷害T細胞と単球の細胞傷害活性

病毒感染诱导的 Gimbuna 细胞毒性 T 细胞和单核细胞的细胞毒活性

• 发表时间: 2010
• 作者: 杣本智軌;中西照幸;中尾実樹
• 通讯作者: 中尾実樹

ニジマスにおけるアスコルビン酸,アスタキサンチン,βグルカン,および三剤混合の短期高濃度投与効果1.人為感染実験における有効性の検証

抗坏血酸、虾青素、β-葡聚糖及三药合剂短期高浓度给药对虹鳟鱼的影响 1. 人工感染实验有效性验证

• 发表时间: 2011
• 作者: 秋山 諭;山下 洋他;中島紀一;桂英・橋本卓爾・藤田武弘・山尾正博・細野賢治;石川孝典・横塚哲也・難波亜紀・藪健史・間野伸宏・澤田守伸・中西照幸
• 通讯作者: 石川孝典・横塚哲也・難波亜紀・藪健史・間野伸宏・澤田守伸・中西照幸