Structural analysis of zoonosis-related high-molecular protein aggregation by double electron-electron resonance (DEER) technique

点击翻译按钮获取中文摘要

In this study, to obtain the structural information for this pathological fibrous form of PrPs', we measured the intermolecular distance in the pathological fibrous form with D178N mutation by the site-directed spin labeling (SDSL) with continuous wave (CW) ESR (X-band) and pulsed ESR (Q-band). After the formation of the fibrous structure by exposure to 1M of guanidine hydrochloride in pH4.0, the ESR spectra were measured by CW-ESR. The interspin-distances in pathological fibrous form were measured by continuous wave ESR (CW-ESR) and the four-pulse double electron-electron resonance (DEER) technique. The information of intermolecular distances will be useful to estimate the pathogenic fibrous structure of the familial prion disease, since it is difficult to analyze the macromolecules such as the fibrous protein by NMR or X-ray crystallography.