Screening, identification and forensic application of age-related biomolecules

1.为了寻找年龄依赖性表达的基因，用靶向Rhit或M-LP的siRNA转染细胞。在64个基因的表达中观察到统计学显著性改变。2. RhitH被证实参与M-LPH的转录调控。3.在RhitH基因的顺式调控元件进行了搜索，并确定了两个转录因子：FOXD 3作为负调控元件，和GABP，线粒体电子传递系统的关键调节器之一，作为正调控元件。4. M-LPH的功能是保护细胞免受氧化应激和/或在应激条件下启动线粒体凋亡级联反应。5.建立了Gpx和RhitH基因SNP分型的简单方法。6.分析成年和胎儿组织中RhitH和M-LPH的mRNA水平。成人组织中M-LPH水平明显高于胎儿组织。

1. To search for age-dependently expressed genes, cells were transfected with siRNA duplexs targeting either Rhit or M-LP. Statistically significant alteration was observed in the expression of sixty four genes. 2. RhitH was confirmed to be involved in transcriptional regulation of M-LPH. 3. Cis-regulatory elements in the RhitH gene were searched, and two transcription factors were identified: FOXD3 as a negative regulatory element, and GABP, one of the key regulators of the mitochondrial electron transport system, as a positive regulatory element. 4. M-LPH functions to protect cells from oxidative stress and/or initiation of the mitochondrial apoptotic cascade under stressed conditions. 5. Simple methods for genotyping of SNPs in the Gpx and RhitH genes were established. 6. The mRNA levels of RhitH and M-LPH in adult and fetal tissues were analyzed. The M-LPH levels in adult tissues were much higher than those in fetal tissues.