Screening, identification and forensic application of age-related biomolecules

1.为了寻找与年龄相关的表达基因，将针对RHIT或M-LP的siRNA双链导入细胞。64个基因的表达有统计学意义的变化。2.RhitH参与了M-LPH的转录调控。3.搜索RhitH基因中的顺式调控元件，鉴定出两个转录因子：FOXD3为负调控元件，GABP为正调控元件。4.M-LPH在应激条件下保护细胞免受氧化应激和/或启动线粒体凋亡级联反应的影响。5.建立了GPX和RhitH基因SNPs的简便基因分型方法。6.检测成体和胎儿组织中RhitH和M-LPH基因的表达水平。成人组织中M-LPH水平明显高于胎儿组织。

1. To search for age-dependently expressed genes, cells were transfected with siRNA duplexs targeting either Rhit or M-LP. Statistically significant alteration was observed in the expression of sixty four genes. 2. RhitH was confirmed to be involved in transcriptional regulation of M-LPH. 3. Cis-regulatory elements in the RhitH gene were searched, and two transcription factors were identified: FOXD3 as a negative regulatory element, and GABP, one of the key regulators of the mitochondrial electron transport system, as a positive regulatory element. 4. M-LPH functions to protect cells from oxidative stress and/or initiation of the mitochondrial apoptotic cascade under stressed conditions. 5. Simple methods for genotyping of SNPs in the Gpx and RhitH genes were established. 6. The mRNA levels of RhitH and M-LPH in adult and fetal tissues were analyzed. The M-LPH levels in adult tissues were much higher than those in fetal tissues.