Screening, identification and forensic application of age-related biomolecules

年龄相关生物分子的筛选、鉴定和法医学应用

• 批准号: 21390215
• 负责人: IIDA REIKO
• 金额: $ 10.82万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390215/
• 关键词: 法医学; 遺伝子発現; 年齢推定; 老化

1. To search for age-dependently expressed genes, cells were transfected with siRNA duplexs targeting either Rhit or M-LP. Statistically significant alteration was observed in the expression of sixty four genes. 2. RhitH was confirmed to be involved in transcriptional regulation of M-LPH. 3. Cis-regulatory elements in the RhitH gene were searched, and two transcription factors were identified: FOXD3 as a negative regulatory element, and GABP, one of the key regulators of the mitochondrial electron transport system, as a positive regulatory element. 4. M-LPH functions to protect cells from oxidative stress and/or initiation of the mitochondrial apoptotic cascade under stressed conditions. 5. Simple methods for genotyping of SNPs in the Gpx and RhitH genes were established. 6. The mRNA levels of RhitH and M-LPH in adult and fetal tissues were analyzed. The M-LPH levels in adult tissues were much higher than those in fetal tissues.

1. 为了寻找与年龄相关的表达基因，用靶向Rhit或M-LP的siRNA双链转染细胞。64个基因的表达有统计学意义的改变。2. RhitH被证实参与M-LPH的转录调控。3. 搜索RhitH基因中的顺式调控元件，确定了两个转录因子：FOXD3为负调控元件，GABP为正调控元件，GABP是线粒体电子传递系统的关键调控因子之一。4. M-LPH的功能是保护细胞免受氧化应激和/或应激条件下线粒体凋亡级联的启动。5. 建立了Gpx和RhitH基因snp分型的简单方法。6. 分析成人和胎儿组织中RhitH和M-LPH mRNA水平。成体组织中M-LPH水平明显高于胎儿组织。

项目成果

Identification of a novel transcriptional repressor, Rhit, involved in heat-inducible and age-dependent expression of Mpv 17-like protein

鉴定出一种新型转录抑制因子 Rhit，它参与 Mpv 17 样蛋白的热诱导和年龄依赖性表达

• 发表时间: 2011
• 作者: Shinkai;S;R. Iida et al
• 通讯作者: R. Iida et al

年齢依存性を示す新規なマウス転写抑制因子Rhitの同定

新型年龄依赖性小鼠转录抑制因子 Rhit 的鉴定

• 发表时间: 2011
• 作者: 安田 年博;飯田 礼子;他
• 通讯作者: 他

A Novel Transcriptional Repressor, Rhit, Is Involved in Heat-Inducible and Age-Dependent Expression of Mpv17-Like Protein, a Participant in Reactive Oxygen Species Metabolism

• DOI: 10.1128/mcb.01025-09
• 发表时间: 2010-05-15
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Iida, Reiko;Ueki, Misuzu;Yasuda, Toshihiro
• 通讯作者: Yasuda, Toshihiro

DNASE Iexon 内SNP検索:アジア人において DNase I Gln222Argのみが多型性を有する

DNASE Iexon 内的 SNP 搜索：只有 DNase I Gln222Arg 在亚洲人中具有多态性

• 发表时间: 2009
• 作者: 藤原純子;高塚尚和;竹下治男;神田芳郎;副島美貴子;小湊慶彦;田島裕;中島たみ子;飯田礼子;安田年博
• 通讯作者: 安田年博

年齢依存性を示す新規な転写抑制因子Rhitの同定

新型年龄依赖性转录抑制因子 Rhit 的鉴定

• 发表时间: 2011
• 作者: 飯田礼子;他
• 通讯作者: 他