A study on regulatory genomic regions and functional RNAs in pancreatic β-cells

为了阐明β细胞转录调控的分子机制，本研究以大鼠β细胞系INS-1D细胞为样本，采用Vector-capping方法构建了新的全长cDNA文库，并对26,976个克隆进行了5′-1过的散弹枪测序，得到了6,042个簇。我们发现了一些基因的新的转录起始位点（tss）和可能的转录调控区域。我们还获得了几种可能参与胰岛素分泌的功能性非编码RNA （ncRNA）候选者。因此，该系统可作为研究胰腺β细胞“RNA世界”的新工具。

Insulin producing pancreatic β-cells are highly differentiated tissue, In order to elucidate molecular mechanisms of transcriptional regulation in β-cells, we constructed a novel full-length cDNA library by Vector-capping method from rat β-cell line INS-1D cells, and performed a 5'-one pass shotgun sequencing with 26,976 clones, resulting in 6,042 clusters. We identified novel transcriptional start sites (TSSs) and possible transcriptional regulatory regions for some genes. We also obtained several functional non-coding RNA (ncRNA) candidates, which may be involved in insulin secretion. Thus, our system should serve as a novel tool for investigating 'RNA world' of pancreatic β-cells.