A study on regulatory genomic regions and functional RNAs in pancreatic β-cells

胰腺β细胞是一种高度分化的胰岛β细胞，为了阐明胰岛β细胞转录调控的分子机制，我们采用Vector-capping方法构建了大鼠胰岛β细胞株INS-1D的全长cDNA文库，并对26，976个克隆进行了5 '端一次鸟枪测序，得到6，042个簇。我们确定了新的转录起始位点（TSS）和可能的转录调控区的一些基因。我们还获得了几个功能性非编码RNA（ncRNA）候选者，这可能涉及胰岛素分泌。因此，我们的系统应该作为一个新的工具，研究胰腺β细胞的“RNA世界”。

Insulin producing pancreatic β-cells are highly differentiated tissue, In order to elucidate molecular mechanisms of transcriptional regulation in β-cells, we constructed a novel full-length cDNA library by Vector-capping method from rat β-cell line INS-1D cells, and performed a 5'-one pass shotgun sequencing with 26,976 clones, resulting in 6,042 clusters. We identified novel transcriptional start sites (TSSs) and possible transcriptional regulatory regions for some genes. We also obtained several functional non-coding RNA (ncRNA) candidates, which may be involved in insulin secretion. Thus, our system should serve as a novel tool for investigating 'RNA world' of pancreatic β-cells.