Clarification of whole scheme of developmental pathway in hematopiesis by fate mapping using a genetic marking system

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We have previously proposed a model of hematopoiesis in which myeloid potential is retained along with development towards T, B and erythroid lineages. In this research project, we aimed to validate this model. Validation of myeloid-B progenitors in fetal hematopoiesis by a single cell analysis was completed(to be submitted). Validation of myeloid-T progenitor stage was achieved by fixing myeloid-T progenitors using a developmental arrest-inducing culture system(Ikawa et al, Science, 2010). Fate map approaches using genetic marking mouse have not yet completed, but a useful line(Flt3-ER-Cre mouse) has been successfully made, and preliminary experiment has provided some promising data. Collectively, the project has been very successful.