Regulation of the delayed-type xenograft rejection and pig retrovirus

本文分析了糖抗原和细胞因子对异种移植排斥反应的调控作用。此外，还对猪内源性逆转录病毒进行了研究.将猪胰岛糖抗原与人胰岛糖抗原进行比较.克隆了猪CMAH基因，并构建了打靶载体.克隆了猪派-2基因，并建立了人FasL修饰基因。研究了糖基化相关基因的敲低对PERV的影响。

For the purpose of the regulating the rejections in xenotransplantation, the carbohydrate antigens and the cellular factors was analyzed. In addition, porcine endogenous retrovirus was also studied.1. The carbohydrate antigen of pig islets was compared with those of humans.2. Pig CMAH gene was cloned, and the targeting vector was prepared.3. Pig PAI-2 gene was cloned, and human modified-FasL gene was also established. The effect of the knockdown of the related gene to glycosylation on PERV was studied.