Regulation of the delayed-type xenograft rejection and pig retrovirus

延迟型异种移植排斥和猪逆转录病毒的调控

• 批准号: 21390361
• 负责人: MIYAGAWA Syuji
• 金额: $ 11.23万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390361/
• 关键词: 糖鎖抗原; レクチン・アレ; ブタ血管内皮; PAI-2; Dol-P-Mannosidase; N型糖鎖; REPV; PERV

For the purpose of the regulating the rejections in xenotransplantation, the carbohydrate antigens and the cellular factors was analyzed. In addition, porcine endogenous retrovirus was also studied.1. The carbohydrate antigen of pig islets was compared with those of humans.2. Pig CMAH gene was cloned, and the targeting vector was prepared.3. Pig PAI-2 gene was cloned, and human modified-FasL gene was also established. The effect of the knockdown of the related gene to glycosylation on PERV was studied.

为了调节异种移植中的排斥反应，对碳水化合物抗原和细胞因子进行了分析。此外，还对猪内源性逆转录病毒进行了研究。 1．猪胰岛糖抗原与人胰岛糖抗原的比较。 2．克隆猪CMAH基因，并制备靶向载体。 3．克隆了猪PAI-2基因，并建立了人修饰的FasL基因。研究了敲低糖基化相关基因对PERV的影响。

项目成果

A cloning of cytidine monophospho-N-acetylneuraminic acid(CMP-NeuAc) hydroxylase from porcine endothelial cell

猪内皮细胞胞苷单磷酸-N-乙酰神经氨酸(CMP-NeuAc)羟化酶的克隆

• 发表时间: 2012
• 期刊: Transplant Proc
• 作者: uke Ikeda;Aki Yamamoto;Akiko Nanjo;Chikako Inuinaka;Yuichi Takama;Takehisa Ueno;Masahiro Fukuzawa;Kazuaki Nakano;Hitomi Matsunari;Hiroshi Nagashima and Shuji Miyagawa.
• 通讯作者: Hiroshi Nagashima and Shuji Miyagawa.

ブタ膵島の糖鎖抗原について

关于猪胰岛糖链抗原

• 发表时间: 2012
• 作者: 王丹丹;高間勇一;上野豪久;福沢正洋;宮川周士;(東京大学)興津輝;(米ベイラー大)松本慎一;(東北大学)後藤昌史;(明治大学)長嶋比呂志
• 通讯作者: (明治大学)長嶋比呂志

A cloning of cytidine monophospho-N-acetylneuraminic acid (CMP-NeuAc) hydroxylase from porcine endothelial cells

猪内皮细胞胞苷单磷酸-N-乙酰神经氨酸 (CMP-NeuAc) 羟化酶的克隆

• 发表时间: 2011
• 作者: Kosuke Ikeda;Akiko Nanjyo;Hiroaki Kashiwada;Yuichi Takama;Takehisa Ueno;Masahiro Fukuzawa;Kazuaki Nakano;Hitomi Matsunari;Hiroshi Nagashima;Shuji Miyagawa
• 通讯作者: Shuji Miyagawa

Complement activation on the xeno-cells Seoul Forum on Xenotransplantation

异种细胞上的补体激活 首尔异种移植论坛

• 发表时间: 2010
• 作者: Sawada Y;Hino R;Hama K;Nakamura M;Tokura Y.;佐野聖三;岩崎寛智;Shuji Miyagawa
• 通讯作者: Shuji Miyagawa

Xenotransplantation

• DOI: 10.1096/fasebj.8.14.7958617
• 发表时间: 1994-11
• 期刊: The FASEB Journal
• 作者: Christopher Y. Lu;T. Khair‐El‐Din;I. Dawidson;T. Butler;Kathleen M. Brasky;Miguel A. Vazquez;S. C. Sicher