Human Umbilical Cord Blood-derived Mononuclear Cell Transplantation Improves Pulmonary Arterial Hypertension in Immunodeficiency Mouse

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Backgrounds and Objectives. The purpose of this study is to investigate whether human umbilical cord blood-derived mononuclear cell(hUCB-MNC) transplantation improves the development of pulmonary arterial hypertension(PAH) in monocrotaline(MCT)-treated mice. HUCB-MNC transplantation may have a new therapeutic potential for several congenital heart defects with primitive pulmonary vasculatures. Methods. MCT-induced PAH model was made using BALB/ c Slc-nu immunodeficiency mouse. HUCB-MNCs were adjusted to 1 x 107/ 0. 25 mL of concentration, and injected intravenously into those mice 4 weeks after MCT administration. The mice were divided into five groups :(1) vehicle-injected mice ;(2) MCT-treated mice ;(3) hUCB-MNC transplantation for PAH mice ;(4) hUCB-MNC+ plasma transplantation for PAH mice ;(5) plasma transplantation for PAH mice. Assessment of PAH were performed by the ratio of right ventricular to ventricular septum plus left ventricular weight(RV/ S+ LV), medial thickness of small pulmonary arteries, and pulmonary acceleration time/ ejection time(PAT/ ET) of the main pulmonary artery by echocardiography. Results. RV/ S+ LV ratio, medial thickness of small pulmonary arteries and PAT/ ET were significantly improved by hUCB-MNC transplantation with or without plasma, respectively. Conclusions. Our data shows that hUCB-MNC transplantation improves MCT-induced PAH and its efficacy is dependent on hUCB-MNCs themselves